江西农业大学学报2024，Vol.46Issue(2)：481-489,9.DOI:10.3724/aauj.2024044
聚乙烯高效降解复合菌系的构建及其降解特性研究
Construction and degradation characteristics of high-efficiency polyethylene degrading composite microbial community
摘要
Abstract
[Objective]The The stability of polyethylene plastics and the increasing usage pose a serious threat of"white pollution"to the ecological environment.The generated microplastics from its weathering poses a significant challenge to biological health and safety.These challenges are in need of urgent solution.[Method]Potential microbial degradation resources for polyethylene in the digestive tract of the greater wax moth larvae were isolated using pure cultivation techniques.An efficient degradation composite microbial community was constructed through the proportional combination of strains.The degradation capacity was assessed through quality control recovery,and its performance was validated by light microscopy and scanning electron microscopy.Microbial degradation characteristics of polyethylene were explored by techniques including infrared spectroscopy,gel permeation chromatography,gas chromatography-mass spectrometry,and liquid chromatography-mass spectrometry.[Result]Thirteen strains of polyethylene-degrading microorganisms were isolated.Debaryomyces hansenii was identified for the first time as having the ability to efficiently degrade polyethylene.A polyethylene efficient degradation composite microbial community composed of fungi and bacteria was established.Compared with single strains,the degradation performance of the composite microbial community increased by 3.3 times,with a degradation efficiency of 0.936 7 mg/d,which significantly surpassed previous levels.Light microscopy and scanning electron microscopy revealed the presence of biofilm traces and noticeable pores on the surface of degraded polyethylene films.Infrared spectroscopy showed the presence of multiple new functional groups in the degradation solution,and gel permeation chromatography results indicated a reduction in the molecular weight of polyethylene microplastics after degradation.Gas and liquid chromatography-mass spectrometry results indicated the presence of bioactive components such as amides,amino acids,olefins,alcohols,ketones,and large-molecule alkanes in the degradation solution.[Conclusion]The study identified microbial degradation resources for polyethylene by constructing an efficient degradation composite microbial community,and it explored microbial degradation characteristics,thus providing data support and feasible solutions for the microbial degradation of polyethylene.关键词
大蜡螟幼虫/复合菌系/聚乙烯/生物降解/降解特性Key words
Galleria mellonella larvae/omposite microbial community/polyethylene/biodegradation/degradation characteristics分类
农业科技引用本文复制引用
李泽霖,徐子航,徐露卉,张庆华..聚乙烯高效降解复合菌系的构建及其降解特性研究[J].江西农业大学学报,2024,46(2):481-489,9.基金项目
国家自然科学基金项目(32160027)和江西省自然科学基金重点项目(20212ACB205003) Project supported by the National Natural Science Foundation of China(32160027)and The Key Project of Natu-ral Science Foundation of Jiangxi Province,China(20212ACB205003) （32160027）
