农业生物技术学报2024，Vol.32Issue(5)：995-1007,13.DOI:10.3969/j.issn.1674-7968.2024.05.002
基于BSA-seq和植物激素联合分析筛选番茄耐热基因
Screening of Heat Tolerance Genes in Tomato(Solanum lycopersicum)Based on BSA-seq and Plant Hormone Combined Analysis
摘要
Abstract
High temperatures affect the physiology and hormone metabolism of tomatoes(Solanum lycopersicum).Localization of genes involved in heat tolerance in tomato can provide theoretical and technical support for subsequent gene cloning and functional analysis.In this study,electrical conductivity was used as the identification index,and 2 tomato strains at seedling stage were identified by high temperature and heat resistance,and the F2 generation isolated population formed by heat-resistant strain CH and heat-sensitive strain DH and their F1 generation were used as materials to detect plant hormones in parents CH and DH.The F2 generation isolated population was resequenced by bulked sergeant analysis(BSA),and candidate genes related to tomato heat resistance were screened through the combined analysis.The results showed that 4 metabolic pathways related to abiotic stress were obtained by phytohormone differential metabolite screening and KEGG annotation,namely glycine,serine and threonine metabolic pathways,amino acid biosynthetic pathway,tryptophan metabolic pathway and aminoacyl-tRNA biosynthetic pathway;6.32 Mb size candidate interval was obtained on chromosome 3 by BSA-seq sequencing.which included 752 candidate genes.Combined with the differential metabolic pathways,5 candidate genes associated with heat tolerance in tomato were finally screened within the candidate regional metabolic pathways,namely:D-glycerate 3-kinase(DG3K),Hydroxypyruvate reductase(HYDR),prolyl-tRNA synthetase(PARS2)and ribulose phosphate 3-epimerase(RPE),aldehyde dehydrogenase(ALDH).The expression of the candidate genes was analysed by qPCR,the expression of candidate genes DG3K,PARS2,and RPE increased extremely significant after 3 hours of high temperature stress(P＜0.01),it was hypothesized that these 3 candidate genes might play an important role in the regulation of heat tolerance in tomato.This study has important implications for molecular assisted selection of heat-resistant tomato lines.关键词
番茄/耐热鉴定/植物激素/混合分离群分析(BSA)Key words
Tomato/Heat resistance identification/Plant hormone/Bulked sergeant analysis(BSA)分类
农业科技引用本文复制引用
姜悦畅,段金虎,李毅丰,毛秀杰,王帅,张宁..基于BSA-seq和植物激素联合分析筛选番茄耐热基因[J].农业生物技术学报,2024,32(5):995-1007,13.基金项目
河北省科技厅现代种业科技专项(20326325D) （20326325D）
