番茄β-BAM基因家族全基因组鉴定及其盐胁迫和外源植物生长调节剂下的表达分析OA北大核心CSTPCD

β-amylase(BAM)is widely involved in the regulation of various biological processes in plants and responds to various external stimuli such as hormone and abiotic stress.In this study,8 SlBAM members in tomato(Solanum lycopersicum)were identified through bioinformatics analysis,and their physical and chemical properties,gene structure,phylogenetic evolution and collinearity were analyzed.The expression pattern of SlBAM gene under salt stress and fruit development was analyzed by qPCR.The results showed that SlBAM possessed typical Glyco_hydro_14 domain,and most of them were acidic and hydrophilic proteins.Phylogenetic analysis showed that SlBAM could be divided into 6 groups,and the same groups had similar gene structure and motif distribution.The composition of cis-acting elements indicated that the expression of most SlBAM may be related to plant hormones,abiotic stress and light response.The results of collinearity analysis showed that tomato had certain homology with Arabidopsis thaliana BAM gene family,and was more closely related to potato(Solanum tuberosum).qRT-PCR analysis showed that SlBAM were mainly expressed in leaves,but almost not expressed in roots and stems.Under salt stress,the expressions of SlBAM2,SlBAM4 and SlBAM6 were up-regulated(P＜0.05),while the expressions of SlBAM1 and SlBAM3 were down-regulated(P＜0.05).When the concentration of 2,4-epibrassinolide(EBR)solution was 0.1 mol/L,the expression of all genes in green ripening stage was significantly up-regulated(P＜0.05).Under the influence of 1-naphthaleneacetic acid(NAA),all the genes except SlBAM6 were up-regulated and only SlBAM6 was down-regulated.These results provide a reference for further elucidations of the functions of the BAM gene family in tomato in response to abiotic stress and fruit development.