广藿香DXS基因克隆及表达分析OA北大核心CSTPCD
Cloning and Expression Analysis of DXS Gene from Pogostemon cablin(Blanco)Benth.
1-脱氧-D-木酮糖-5-磷酸合酶(DXS)是调控萜类合成途径中MEP途径的第一个关键酶,为探究广藿香DXS基因参与其主要萜类成分广藿香醇合成调控的分子机制,本研究依据本课题组前期获得的转录组DXS基因序列,以广藿香cDNA为模板,克隆得到PcDXS基因,对其进行生物信息学分析,构建pET-28a-PcDXS原核表达载体诱导蛋白表达,并采用荧光实时定量PCR法检测广藿香根、茎、叶、芽中DXS基因表达情况.结果表明,从广藿香叶中克隆到开放阅读框全长…查看全部>>
1-Deoxy-D-xylulose-5-phosphate synthase(DXS)is the first key enzyme to regulate the MEP pathway in the terpene synthesis pathway.In order to illustrate the molecular mechanism of DXS gene partici-pating in synthesis and regulation of the main terpenoid component,patchouli alcohol,in patchouli(Pogoste-mon cablin),the research was conducted based on the transcriptome DXS gene sequence obtained previously by our research group.PcDXS gene was cloned by using pat…查看全部>>
曾晴;严雅玲;严寒静;何梦玲;张宏意
广东药科大学中药学院,广东 广州 510006广东药科大学中药学院,广东 广州 510006广东药科大学中药学院,广东 广州 510006广东药科大学中药学院,广东 广州 510006广东药科大学中药学院,广东 广州 510006
农业科学
广藿香1-脱氧-D-木酮糖-5-磷酸合酶(DXS)基因克隆生物信息学分析表达分析
Pogostemon cablin1-Deoxy-D-xylose-5-phosphate synthase(DXS)Gene cloningBioin-formatics analysisExpression analysis
《山东农业科学》 2024 (4)
28-35,8
广东省中医药局科研项目(20241177)广东省重点领域研发计划项目(2020B020221002)
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