四川中医2024，Vol.42Issue(2)：86-91,6.
基于IRE1α/XBP1/CHOP通路探讨黄连解毒汤对内质网应激的作用机制
Study on the Mechanism of Huanglian Jiedu Decoction on ERS Based on IRE 1α/XBP1/CHOP Pathway
摘要
Abstract
Objective To study the effect of Huanglian Jiedu Decoction(HLJDD)containing serum on endoplasmic reticulum transmembrane kinase 1(IRE1)in the endoplasmic reticulum stress of human umbilical vein endothelial cells(HUVEC)in-duced by tunicamycin α、The effect of X box binding protein(XBP1)and cyclic adenosine monophosphate response element binding transcription factor homologous protein(C/EBP homologous protein,CHOP)on the expression of X box binding protein(XBP1)was studied to explore the cellular pathway mechanism of Huanglian Jiedu decoction on endoplasmic reticulum stress.Methods HLJDD and captopril containing serum were prepared,and the cells were divided into control group,TM group,HLJDD low,medium and high dose groups,and captopril group.The cell survival rate was detected by CCK8method;The level of apop-tosis was detected by flow cytometry.The protein expression of endoplasmic reticulum stress glucose-regulated protein 78kD(GRP78),CHOP,IRE1α,XBP1,PDIA6,eNOS was detected by protein blotting;and the mRNA of GRP78,CHOP,IRE1α,XBP1,PDIA6,eNOS was detected by real-time fluorescent quantitative PCR.Results Results Compared with blank control group,the cell survival rate in model group was decreased,the apoptosis rate was increased,and the expressions of GRP78,CHOP,IRE1α,XBP1and PDIA6were increased(P＜0.05).The expression of eNOS was decreased(P＜0.05).Com-pared with model group,the apoptosis rate and the expressions of GRP78,CHOP,IRE1α,XBP1and PDIA6were decreased sig-nificantly in Huanglian Jiedu decoction group and captopril group(P＜0.05).eNOS expression was increased(P＜0.05).Con-clusion Huanglian Jiedu Decoction can improve ERS status,inhibit cell apoptosis,and protect endothelial cells.The mechanism may be related to the inhibition of IRE1α/XBP1/CHOP signaling pathway.关键词
黄连解毒汤/内质网应激/内皮细胞/信号通路Key words
Huanglian Jiedu Decoction/Endoplasmic reticulum stress/endothelial cell/signaling pathway分类
医药卫生引用本文复制引用
周月,吴福佳,李建橡,张爱珍,贺鸿捷,许育佳,岳桂华..基于IRE1α/XBP1/CHOP通路探讨黄连解毒汤对内质网应激的作用机制[J].四川中医,2024,42(2):86-91,6.基金项目
国家自然科学基金-地区科学基金项目(编号:81860831) （编号:81860831）
广西中医药大学校级研究生科研创新项目(编号:YCSY2023058). （编号:YCSY2023058）
