山东农业科学2024，Vol.56Issue(4)：124-132,9.DOI:10.14083/j.issn.1001-4942.2024.04.016
无子刺梨内生真菌拮抗活性菌株筛选及分子鉴定
Screening and Molecular Identification of Active Endophytic Fungi Strains of Rosa sterilis S.D.Shi
摘要
Abstract
In order to further develop and utilize endophytic fungi with biological control potential in branches and leaves of Rosa sterilis,two species of wood-rotting fungi and four species of plant pathogenic fun-gi were used as target,the endophytic fungi strains with antagonistic activity were screened by five-point con-frontation method,and the classification status of the active strains was discussed.The results showed that the inhibition rate of RSZF 36,RSZF 39 and RSYF 34 against the two species of wood-rotting fungi were all over 80%,and that of RSZF 34 and RSZF 36 against the four species of plant pathogenic fungi were all over 75%.The inhibition rate of RSZF 36 against the 6 species of pathogens were all over 75%,showing strong inhibition ability.Among the 84 endophytic fungi strains,12 were active strains with the inhibition rate higher than 70%against the 6 pathogens,among which,9 from branches and 3 from leaves.The 12 active strains were identi-fied by molecular biology and classified into 1 phylum,2 classes,3 orders,3 families and 4 genera.Pestaloti-opsis was the dominant genus,accounting for 41.67%of the active strains.To sum up,antibacterial strains were widely distributed in the endophytic fungi of R.sterilis,and the antagonistic ability of different strains to different pathogens showed a certain selectivity or specificity.The results could provide theoretical references for further development and utilization of endophytic fungi resources from R.sterilis.关键词
无子刺梨/内生真菌/拮抗活性/筛选/鉴定/植物病原菌Key words
Rosa sterilis/Endophytic fungi/Antagonistic activity/Screening/Identification/Plant path-ogenic fungi分类
农业科技引用本文复制引用
罗福新,阚欢,李永和,赵平,刘云,罗旭璐..无子刺梨内生真菌拮抗活性菌株筛选及分子鉴定[J].山东农业科学,2024,56(4):124-132,9.基金项目
云南省科技计划项目农业联合专项(202301AK070157) （202301AK070157）
