海南热带海洋学院学报2024，Vol.31Issue(2)：11-17,7.DOI:10.13307/j.issn.2096-3122.2024.02.02
杉虎杂交斑5S rRNA基因甲基化分析
Methylation Analysis of 5S rRNA Gene in the Hybrid Grouper(Epinephelus fuscoguttatus × Epinephelus polyphekadion)
摘要
Abstract
Ribosomal RNA(rRNA)is an important component of ribosomal which plays an important role in protein synthesis.The hybrid grouper derived from the hybridization of Epinephelus fuscoguttatus × Epinephelus polyphekadion,exhib-its a certain of advantages in growth and meat quality.The DNA methylation levels of the first 1 000 bp of the coding re-gion of 5S rRNA gene,coding region and non-transcribed spacer of 5S rRNA gene were measured and analyzed contras-tively between the hybrid grouper and its parents.Results showed that the overall methylation level of 5S rRNA gene in the hybrid grouper(0.418)was higher than that of E.fuscoguttatus(0.379)and E.polyphekadion(0.037).While there was no significant difference between the hybrid grouper and E.fuscoguttatus,significant difference was detected between the hybrid grouper and E.polyphekadion.Comparative analysis of DNA methylation level at each CpG site in the coding region and non-transcribed spacer revealed that all methylation sites co-existed in both the hybrid grouper and E.fuscoguttatus,but were not found in E.polyphekadion.These results indicate that the methylation pattern of the 5S rRNA gene in the hy-brid grouper was similar to that of E.fuscoguttatus.The observations elucidate the DNA methylation level and pattern of 5S rRNA gene in the hybrid grouper,and provide a theoretical basis for cross-breeding in grouper.关键词
石斑鱼/杂交/5S rRNA基因/DNA甲基化Key words
grouper/hybridization/5S rRNA gene/DNA methylation分类
农业科技引用本文复制引用
曹柳,黄潇庆,陈菁苗,卢艳,黄海..杉虎杂交斑5S rRNA基因甲基化分析[J].海南热带海洋学院学报,2024,31(2):11-17,7.基金项目
海南热带海洋学院崖州湾创新研究院青年项目(2022CXYQNXM06) （2022CXYQNXM06）
海南省重大科技计划项目(ZDKJ2021017) （ZDKJ2021017）
海南省自然科学基金青年基金项目(321QN263) （321QN263）
海南热带海洋学院引进人才科研启动项目(RHDRC202010) （RHDRC202010）
