海南热带海洋学院学报2024，Vol.31Issue(2)：18-24,7.DOI:10.13307/j.issn.2096-3122.2024.02.03
台湾泥鳅腐皮病霍乱弧菌的分离与鉴定
Isolation and Identification of V.cholerae from the Rotting Skin Disease of Paramisgurnus dabryanus ssp.
摘要
Abstract
In order to study the pathogen of P.dabryanus ssp.,a dominant strain named P1 was obtained by bacterial isolation and purification of its rotting skin and other lesions.The morphological characteristics of the strain were observed by Gram staining,and the sequence of the pathogen was obtained by PCR amplification and 16S rRNA gene sequencing.The phylogenetic tree was constructed by MEGA software.Then the name of the bacterial species was identified through physiological and biochemical analysis.The results showed that Gram staining was negative,curved,and bluntly rounded at both ends;the bacterium was 1-1.5 μm in length,1-1.2 μm in width,with mycelium and flagellum;16S rRNA gene was 1 395 bp;the establishment of the phylogenetic tree revealed that P1 was in the same branch with Vibrio cholerae;the results of physiological and biochemical experi-ments were in agreement with those of Vibrio cholerae,which leads to the identification of the strain as Vibrio cholerae.The results of drug sensitivity test showed that the isolated strain was highly sensitive to nine antibiot-ics,including cefoperazone,canomyces and vancomycin,moderate sensitive to tetracycline,resistant to 18 antibiot-ics,including furazolidone,cotrimoxazole and clindamycin.The results provide reference for the pathogen identifi-cation and drug control of P.dabryanus ssp..关键词
台湾泥鳅/腐皮病/霍乱弧菌/分离/鉴定Key words
Paramisgurnus dabryanus ssp./rotting skin disease/Vibrio cholerae/isolation/identification分类
农业科技引用本文复制引用
杨宁,姜芳燕,陈攀,马军,陈燕,曹柳,侯兴蓉..台湾泥鳅腐皮病霍乱弧菌的分离与鉴定[J].海南热带海洋学院学报,2024,31(2):18-24,7.基金项目
三亚市院地科技合作项目(2016YD20) （2016YD20）
海南热带海洋学院科研启动项目(RHDRC202101) （RHDRC202101）
海南省院士创新平台资金资助项目 （）
