口腔颌面外科杂志2024，Vol.34Issue(2)：100-107,8.DOI:10.12439/kqhm.1005-4979.2024.02.004
口腔鳞状细胞癌E2F转录因子1的表达与临床的意义
Expression and clinical significance of E2F transcription factor 1 in OSCC
摘要
Abstract
Objective:To investigate the expression and clinical significance of E2F transcription factor 1(E2F1)in oral squamous cell carcinoma(OSCC),and to analyze its function in apoptosis and cell cycle.Methods:The correlation between E2F1 expression and clinicopathology in OSCC was analyzed based on R language and The Cancer Genome Atlas(TCGA)database.The main biological processes involved in E2F1 were identified by gene set enrichment analysis(GSEA).Western blotting and real-time quantitative polymerase chain reaction(RT-qPCR)were used to detect E2F1 expression in OSCC patients'tissue.After increasing and knocking down the expression of E2F1 in SCC15 cell lines by cell transfection,the effect of changes in E2F1 expression on apoptosis and cycle of SCC15 cells was analyzed by flow cytometry.Results:E2F1 was highly expressed in the OSCC-related TCGA dataset and correlated with T-stage(T2 or T4 vs T1),histological grade(G2 or G3 vs G1),clinical stage(Ⅲ vs Ⅰ),age(middle vs young),and gender(male vs female)(P＜0.05),which were mainly enriched in the genome of cell cycle or nucleotide excision repair[P＜0.05 and false discovery rate(FDR)＜0.25].Both mRNA and protein expression of E2F1 were upregulated in the tissues of 37 OSCC patients(P＜0.001).The expression of upregulated E2F1 decreased the rate of apoptosis in SCC15 cells(P＜0.05),and also decreased the rate of G1 phase(P＜0.01)and increased the rate of S phase(P＜0.001)in the cell cycle.Conclusion:E2F1 is highly expressed in OSCC and can inhibit apoptosis and promote G1/S phase transition in the cell cycle.关键词
口腔鳞状细胞癌/E2F转录因子1/生物信息学分析/细胞凋亡/细胞周期Key words
oral squamous cell carcinoma/E2F transcription factor 1/bioinformatics analysis/apoptosis/cell cycle分类
医药卫生引用本文复制引用
王锦航,崔子峰,杨凯成,陈彦平,彭士雄..口腔鳞状细胞癌E2F转录因子1的表达与临床的意义[J].口腔颌面外科杂志,2024,34(2):100-107,8.基金项目
河北省卫生厅青年科技课题(20211145) （20211145）
河北省科技计划项目(22377779D) （22377779D）
河北省自然科学基金项目(H2022206410) （H2022206410）
