海南热带海洋学院学报2024，Vol.31Issue(2)：104-112,9.DOI:10.13307/j.issn.2096-3122.2024.02.15
卷柏总黄酮的纯化工艺与细胞保护作用
Purification Process of Total Flavonoids from Selaginella pulvinata and Protecting Effects on Neuroblastoma Cells
摘要
Abstract
Flavonoids,especially biflavonoids,are main components of Selaginella tamariscina and Selaginella pulvinate.The purification process of total flavonoids from Selaginella pulvinata(TFSP)was established by purifying the Selaginella pulvinata extract through AB-8 macroporous resin followed by polyamide.The content of TFSP was determined by ultravio-let spectrophotometry.The protecting and repairing effects of TFSP on hydrogen peroxide induced neuron cell damage were detected by MTT.The results showed that AB-8 macroporous resin adsorbed and removed the liposoluble substance from the extract,and flavonoids were isolated through adsorption-desorption in polyamide column.The content of TFSP yielded from the process was up to 81.4％under the optimal conditions—polyamide resin uploading volume of 1:2(C.matta vs.resin),and 80％ethanol eluent of 5∶1(eluent vs.polyamide column).The purification process is scientific,reasonable,simple and easy to implement.TFSP showed remarkable activity on neuroblastoma cell and increased the cell viability before or after it was cocultured with SH-SY5Y cells.Therefore,TFSP has good effects on nerve cell peroxidation damage with the potential to be developed as therapeutic drug for neurodegenerative disorder.关键词
卷柏总黄酮/纯化/大孔树脂/神经细胞/保护作用Key words
total flavonoids from Selaginella pulvinate/purification/macroporous resin/neuroblastoma cell/protecting effect分类
医药卫生引用本文复制引用
吕雪峰,王莲萍,张明天,张莲珠..卷柏总黄酮的纯化工艺与细胞保护作用[J].海南热带海洋学院学报,2024,31(2):104-112,9.基金项目
海南省自然科学基金高层次人才项目(821RC598) （821RC598）
