山东医药2024，Vol.64Issue(12)：15-19,5.DOI:10.3969/j.issn.1002-266X.2024.12.004
异钩藤碱对大鼠急性胰腺炎细胞的炎症和凋亡改善及ERK信号通路调控作用
Effects of isorhynchophylline on inflammation,apoptosis and ERK signaling pathway of acute pancreatitis cells in rats
摘要
Abstract
Objective To observe the effects of isorhynchophylline(LSO)on inflammation,apoptosis and ERK sig-naling pathway of acute pancreatitis(AP)cells in rats.Methods AR42J cells were divided into six groups.Cells in the control group were cultured normally;cells in the model group,LSO group,inhibitor group,LSO+ inhibitor group and LSO+ activator group were added with 100 nmol/L cerulein to prepare AP cell models,and cells in the LSO group were added with 40 μmol/L LSO after modeling,10 μmol/L U0126 was added to the inhibitor group,40 μmol/L LSO and 10 μmol/L U0126 were added to the LSO+ inhibitor group,and 40 μmol/L LSO and 0.1 μmol/L C16-PAF were added to the LSO+ activator group.Inflammatory factors(TNF-α,IL-6,IL-8,IL-1β),proliferation rate,and apoptosis rate in cell cul-ture medium were determined by ELISA,EdU method and Hoechst 33258 staining,respectively.The mRNA levels of nu-cleotide oligomerization domain-like receptor protein 3(NLRP3),speckle-like protein(ASC)and Caspase-1 in the cells were determined by qPCR,and the protein levels of ERK 1/2,p-ERK 1/2,NLRP3,ASC and Caspase-1 were determined by WB.Results Compared with the control group,TNF-α,IL-6,IL-8,IL-1β,apoptosis rate,p-ERK 1/2 protein,NL-RP3,ASC,Caspase-1 mRNA and protein increased,and the proliferation rate decreased in the model group(all P<0.05).Compared with the model group,inflammatory cytokines(TNF-α,IL-6,IL-8,IL-1β),apoptosis rate,p-ERK 1/2 protein,NLRP3,ASC,Caspase-1 mRNA and protein decreased,and proliferation rate increased in the LSO group and inhibitor group(all P<0.05).Compared with the LSO group,U0126 in the LSO+ inhibitor group enhanced the effect of LSO on the above trend of cells,while C16-PAF in the LSO+ activator group reversed the effect of LSO on the above trend of cells(all P<0.05).Conclusion LSO can inhibit the inflammation and apoptosis of AP cells probably by regulating ERK signaling pathway.关键词
异钩藤碱/急性胰腺炎/炎症/细胞增殖/细胞凋亡/核苷酸寡聚化结构域样受体蛋白3炎症小体/细胞外信号调节激酶通路Key words
isorhynchophylline/acute pancreatitis/inflammation/cell proliferation/apoptosis/nucleotide oligo-merization domain-like receptor protein 3 inflammasome/extracellular signal-regulated kinase pathway分类
医药卫生引用本文复制引用
寨旭,裴红红,刘俊,黄婉..异钩藤碱对大鼠急性胰腺炎细胞的炎症和凋亡改善及ERK信号通路调控作用[J].山东医药,2024,64(12):15-19,5.基金项目
陕西省重点研发计划项目(2022SF-568). （2022SF-568）
