山东医药2024，Vol.64Issue(12)：20-23,4.DOI:10.3969/j.issn.1002-266X.2024.12.005
早发性卵巢功能不全患者外周血Tfh占比、功能相关细胞因子表达变化及其与性激素水平的相关性
Changes in peripheral blood Tfh proportion and expression of function-related cytokines and their correlations with sex hormone levels in patients with POI
摘要
Abstract
Objective To observe the changes in the proportion of peripheral blood T follicular helper(Tfh)cells and the expression of function-related cytokines in patients with premature ovarian insufficiency(POI),and to analyze their correlations with sex hormone levels.Methods We recruited 29 POI patients(observation group)and 31 healthy individuals(control group)for physical examination during the same period.We used flow cytometry to detect the propor-tion of Tfh in whole blood,immunoblotting to measure the expression of CXCR5 and BCL-6 proteins in peripheral blood mononuclear cells(PBMCs),and ELISA to detect serum levels of IL-6 and IL-21.Electrochemiluminescence immunoas-say was employed to determine serum levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH),prolactin,progesterone,testosterone,estradiol(E2),and anti-Mullerian hormone(AMH).Spearman's correlation analysis was per-formed to assess the correlations between Tfh,CXCR5 protein,BCL-6 protein,IL-6,IL-21,and sex hormones.Results Compared with the control group,the observation group exhibited an increased proportion of Tfh in whole blood,elevated serum levels of IL-6 and IL-21,up-regulated expression of CXCR5 and BCL-6 proteins in PBMCs,higher serum levels of FSH and LH,and lower serum levels of E2 and AMH,with statistically significant differences(all P<0.05).The propor-tion of Tfh in whole blood was positively correlated with serum levels of FSH and LH(r =0.75 and 0.63,respectively;both P<0.05),and was negatively correlated with serum levels of E2 and AMH(r=-0.35 and-0.82,respectively;both P<0.05).Similarly,serum IL-6 level was positively correlated with serum FSH and LH levels(r = 0.75 and 0.62,respec-tively;both P<0.05)and was negatively correlated with serum E2 and AMH levels(r=-0.47 and-0.77,respectively;both P<0.05).Additionally,serum IL-12 level was positively correlated with serum FSH and LH levels(r=0.68 and 0.56,respectively;both P<0.05)and was negatively correlated with serum E2 and AMH levels(r=-0.25 and-0.73,respectively;both P<0.05).Expression of BCL-6 protein in PBMCs was positively correlated with serum FSH and LH lev-els(r=0.73 and 0.60,respectively;both P<0.05)and was negatively correlated with serum E2 and AMH levels(r=-0.27 and-0.76,respectively;both P<0.05).Similarly,the expression of CXCR5 protein in PBMCs was positively cor-related with serum FSH and LH levels(r=0.72 and 0.58,respectively;both P<0.05)and was negatively correlated with serum E2 and AMH levels(r=-0.27 and-0.70,respectively;both P<0.05).Conclusions In POI patients,there is an increased proportion of Tfh in whole blood,up-regulated expression of CXCR5 and BCL-6 proteins in PBMCs,elevated serum levels of IL-6,IL-21,FSH,and LH,as well as decreased levels of E2 and AMH.Tfh and its related factors show positive or negative correlations with sex hormones,suggesting that Tfh and its associated factors may be involved in the pathogenesis of POI.关键词
早发性卵巢功能不全/滤泡辅助性T细胞/白细胞介素6/白细胞介素21/CXCR5蛋白/BCL-6蛋白/促卵泡刺激素/促黄体生成素/雌二醇/抗缪勒管激素Key words
premature ovarian insufficiency/T follicular helper(Tfh)cells/interleukin-6/interleukin-21/CX-CR5 protein/BCL-6 protein/follicle-stimulating hormone/luteinizing hormone/estradiol/anti-Mullerian hormone分类
医药卫生引用本文复制引用
麦尔哈巴·艾斯卡尔,朱玥洁,张曼丽,张于念,巩晓芸,王辉,丁剑冰..早发性卵巢功能不全患者外周血Tfh占比、功能相关细胞因子表达变化及其与性激素水平的相关性[J].山东医药,2024,64(12):20-23,4.基金项目
省部共建国家重点实验室开放课题(SKL-HIDCA-2022-JZ6) （SKL-HIDCA-2022-JZ6）
河南省免疫与靶向药物重点实验室开放课题(HNKLITD2022001). （HNKLITD2022001）
