陕西中医2024，Vol.45Issue(5)：579-582,587,5.DOI:10.3969/j.issn.1000-7369.2024.05.001
丹黄散对糖尿病小鼠皮肤创面愈合的促进作用及调节细胞自噬机制
Promoting effect of Danhuang powder on skin wound healing in diabetic mice and its mechanism of regulating autophagy
摘要
Abstract
Objective:To investigate the effect of Danhuang powder on the expression of autophagy protein LC3/Beclin1 during ulcer wound healing in diabetic mice.Methods:18 db/db mice with spontaneous type 2 diabetes were randomly divided into model group,growth factor group and Danhuang powder group,and 6 db/m mice were included in the blank group and model group.After the diabetes wound model was prepared,the blank group and model group were only debrideed without drug treatment,and the growth factor group and Danhuang powder group were treated with the corresponding drug after debridement,once a day for 21 days.Wound healing,wound pathology with HE staining,mitochondria and autophagosomes under transmission electron microscopy,and LC3/Beclin1 ex-pression in wound tissue were detected by immunohistochemical method at 7,14 and 21 days after the molding.Results:The wound healing rate of 7,14,21 days blank group,growth factor group and Danhuang powder group was higher than that of model group(P<0.05).HE staining results showed that compared with model group and blank group,Danhuang powder group had higher increase of neovascularization and fibroblasts,the expression of LC3 and Beclin1 protein in wound tissue of mice in blank group,growth factor group and Danhuang powder group was en-hanced by immunohistochemistry.Conclusion:Danhuang powder may improve the healing effect of ulcer wound in mice by up-regulating LC3/Beclin1 expression.关键词
糖尿病/丹黄散/创面愈合/细胞自噬/自噬相关蛋白管连接蛋白轻链3/苄氯素1/小鼠Key words
Diabetes mellitus/Danhuang powder/Wound healing/Autophagy/LC3/Beclin1/Mice分类
医药卫生引用本文复制引用
董源源,赵思思,罗智钦,张春玲,赵伟,魏良纲,陈露,邸铁涛,王艳辉,范燚,朱蕾..丹黄散对糖尿病小鼠皮肤创面愈合的促进作用及调节细胞自噬机制[J].陕西中医,2024,45(5):579-582,587,5.基金项目
国家自然科学基金资助项目(81960805) （81960805）
贵州省教育厅创新群体重大研究项目[黔教合KY字(2017)042] （2017）
