丹黄散对糖尿病小鼠皮肤创面愈合的促进作用及调节细胞自噬机制OACSTPCD

Objective:To investigate the effect of Danhuang powder on the expression of autophagy protein LC3/Beclin1 during ulcer wound healing in diabetic mice.Methods:18 db/db mice with spontaneous type 2 diabetes were randomly divided into model group,growth factor group and Danhuang powder group,and 6 db/m mice were included in the blank group and model group.After the diabetes wound model was prepared,the blank group and model group were only debrideed without drug treatment,and the growth factor group and Danhuang powder group were treated with the corresponding drug after debridement,once a day for 21 days.Wound healing,wound pathology with HE staining,mitochondria and autophagosomes under transmission electron microscopy,and LC3/Beclin1 ex-pression in wound tissue were detected by immunohistochemical method at 7,14 and 21 days after the molding.Results:The wound healing rate of 7,14,21 days blank group,growth factor group and Danhuang powder group was higher than that of model group(P<0.05).HE staining results showed that compared with model group and blank group,Danhuang powder group had higher increase of neovascularization and fibroblasts,the expression of LC3 and Beclin1 protein in wound tissue of mice in blank group,growth factor group and Danhuang powder group was en-hanced by immunohistochemistry.Conclusion:Danhuang powder may improve the healing effect of ulcer wound in mice by up-regulating LC3/Beclin1 expression.