青天葵总黄酮对人鼻咽癌CNE-2荷瘤裸鼠皮下肿瘤的抑制作用OACSTPCD

Objective:The regulatory effect of Total Flavonoids from Nervilia fordii(TFNF)on the TGF-β1/Erk/MAPK pathway in the subcutaneous transplantation model of human nasopharyngeal cancer cells in BALB/c nude mice was investigated.Methods:BALB/c nude mice were inoculated with CNE-2 cells(1.0×107cells/ml)to es-tablish a tumor-bearing model of nasopharyngeal carcinoma,the pathological determination was performed by hema-toxylin-eosin(HE),and the expression of cell cycle regulatory protein-67(Ki67)and proliferation nuclear antigen(PCNA)were observed by immunohistochemistry(IHC).Western blotting detected the protein expression of trans-forming growth factor-β1(TGF-β1),extracellular regulated protein kinase(Erk),p-Erk,mitogen-activated protein kinase(MAPK),and p-MAPK.Superoxide dismutase(SOD)and malondialdehyde(MDA)levels in the kidneys,liv-er,and spleen were assessed by the kit.Results:Compared with the model group,high-dose TFNF significantly inhibi-ted tumor volume and weight,significantly reduced the expression levels of Ki67(55.19%)and PCNA(46.65%),increased SOD levels in kidneys,liver,and spleen(to 3.88,2.98,3.71 U/mg prot),and decreased MDA levels in kid-neys,liver,and spleen(to 2.19,1.05,2.85 nmol/mg prot).In addition,TFNF can also reduce the protein expression levels of TGF-β1(47.36%),p-Erk/Erk(81.13%),and p-MAPK/MAPK(19.80%)in tumor tissues.Conclusion:The re-sults showed TFNF could regulate the TGF-β1/Erk/MAPK pathway,inhibit tumor growth,and reduce the occurrence of oxidative damage to the main organs of tumor-bearing nude mice.