生态学杂志2024，Vol.43Issue(4)：1082-1091,10.DOI:10.13292/j.1000-4890.202403.015
转抗草甘膦基因甘蓝型油菜根际土壤理化性质及真菌群落多样性
Physicochemical properties and fungal community diversity in rhizosphere soil of transgenic glyphosate-resistant Brassica napus
摘要
Abstract
In the study of environmental safety assessment of transgenic crops,whether the introduction of exoge-nous genes will affect the ecological balance of soil microbial populations and non-target organisms has been contro-versial.In this study,we analyzed the differences in rhizosphere soil physicochemical properties of glyphosate-resistant Brassica napus overexpressing mEPSPS(JHX6)(GMO)and the control material(J9707)(GMO-free)in different developmental stages.The fungal communities of rhizosphere soil in field and pot experiments were ana-lyzed using ITS high-throughput sequencing technology of IonS5TMXL.Results showed that there was no significant difference in physicochemical properties of rhizosphere soil between the two materials at the same developmental sta-ges.The main fungal phyla enriched in the rhizosphere soil of B.napus were Olpidiomycota and Ascomycota.There were no significant differences of alpha diversity and structure of fungal community in the rhizosphere soil of both GMO and GMO-free at the same developmental stages.There was a significant difference in the relative abundance of Olpidiomycota in the rhizosphere soil of the two materials in both pot and field experiments.Our results suggest that the mEPSPS introduction in B.napus did not affect rhizosphere soil physicochemical properties and fungal com-munity structure and diversity,but would affect the relative abundance of specific fungal species.关键词
mEPSPS基因/甘蓝型油菜/根际/真菌多样性/群落结构Key words
mEPSPS gene/Brassica napus/rhizosphere/fungal diversity/community structure引用本文复制引用
杜坤,李金萍,王婷,张晶晶,王明,王幼平..转抗草甘膦基因甘蓝型油菜根际土壤理化性质及真菌群落多样性[J].生态学杂志,2024,43(4):1082-1091,10.基金项目
转基因生物新品种培育重大专项(2018ZX08020001-012)资助. （2018ZX08020001-012）
