中国临床药理学杂志2024，Vol.40Issue(8)：1160-1164,5.DOI:10.13699/j.cnki.1001-6821.2024.08.015
姜黄素通过抑制炎症因子IL-6调控胃肠道间质瘤细胞的增殖抑制的研究
Curcumin regulates the proliferation inhibition of gastrointestinal stromal tumor cells by inhibiting the inflammatory factor IL-6
摘要
Abstract
Objective To investigate whether curcumin is a potential drug for the treatment of gastrointestinal stromal tumors(GIST).Methods The differential genes of imatinib-resistant cells and non-resistant cells were analyzed by cell transcriptology.The antitumor activity of curcumin was verified by cell counting kit-8(CCK-8)method,and the concentration of Curcumin ranged from 5 to 80 μg·mL-1for GIST-T1 and GIST-T1/IMR cells.20 μg·mL-1 Curcumin as the experimental group,phosphate buffered solution as the control group.The contents of interleukin-6(IL-6),reactive oxygen species(ROS)and nitric oxide(NO)were measured by enzyme linked immunosorbent assay.The cell cycle changes were analyzed by flow cytometry.Results Using non-resistant cells as a contrast,the results showed that there were 1 300 up-regulated genes and 1 609 down-regulated genes in imatinib-resistant cells.The 50％inhibiting concentration values of Curcumin on GIST-T1 and GIST-T1/IMR cells were(15.33±1.36)and(10.49±2.12)μg·mL-1,respectively.In GIST-T1 cells,the IL-6 levels in experimental group and control group were(3.45±0.01)and(5.64±0.42)pg·mL-1;the ROS levels were(2 841.42±81.83)and(4 174.32±439.12)pg·mL-1;the iNOS levels were(7.02±0.08)and(8.08±0.03)μmol·L-1,respectively.In GIST-T1/IMR cells,the IL-6 levels in experimental group and control group were(2.47±0.30)and(6.30±0.01)pg·mL-1;the ROS levels were(4 706.40±146.71)and(8 254.34±342.35)pg·mL-1;the iNOS levels were(6.42±0.09)and(7.29±0.04)μmol·L-1,respectively.Among the 2 cells,the differences of above indicators were statistically significant between the experimental group and the control group(P＜0.05,P＜0.01).Curcumin blocked the cell cycle of GIST-T1 and GIST-T1/IMR in G1 phase,further shortens S phase and G2 phase.Conclusion Curcumin can inhibit the secretion of inflammation and regulate the proliferation of GIST.关键词
姜黄素/炎症/白细胞介素-6/胃肠道间质瘤/细胞增殖Key words
Curcumin/inflammation/interleukin-6/gastrointestinal stromal tumor/cell proliferation分类
医药卫生引用本文复制引用
陈燕,李雨珂,王茹静,肖洪涛,石三军..姜黄素通过抑制炎症因子IL-6调控胃肠道间质瘤细胞的增殖抑制的研究[J].中国临床药理学杂志,2024,40(8):1160-1164,5.基金项目
中国药学会医院药学专业委员会科研专项基金资助项目(CPA-Z05-ZC-2022-002) （CPA-Z05-ZC-2022-002）
四川省肿瘤医院青年优秀基金资助项目(YB2023026) （YB2023026）
四川省抗癌协会临床科研基金资助项目[(齐鲁)HX2023-自由探索002] （齐鲁）
