中国临床药理学杂志2024，Vol.40Issue(8)：1189-1193,5.DOI:10.13699/j.cnki.1001-6821.2024.08.021
济川煎对慢传输型便秘大鼠肠道功能的影响及作用机制的研究
Effect and mechanism of Jichuan decoction on intestinal function in rats model of slow transit constipation
摘要
Abstract
Objective To investigate the effect of Jichuan decoction on defecation in rats with slow transit constipation(STC).Methods A total of 36 SD rats were divided into model group,experimental group and control group.Except the control group,STC rat models were established in the other two groups by gavage of 2％rhein acid suspension.The experimental group was given 170％Jichuan decoction of 10 mL·kg-1 by gavage,while the control group and model group were given with 0.9％NaCl by gavage,once a day for 30 days.Fecal dry-wet weight ratio and colon transport time were observed by fecal weighing and recording of first fecal discharge time;substance P(SP)and vasoactive peptide(VIP)contents in plasma were detected by kit;changes in intestinal flora were detected by real-time fluorescent quantitative polymerase chain reaction(qRT-PCR).Results The dry-wet ratio of feces in control group,model group and experimental group were(44.86±4.40)％,(53.44±3.52)％and(44.28±4.33)％,respectively;the colon transport time were(340.17±34.91),(424.67±37.26)and(377.36±38.87)min,respectively;plasma SP were(79.86±7.56),(60.30±2.41)and(78.84±5.83)pg·mL-1,respectively;plasma VIP were(30.56±2.87),(23.38±6.62)and(28.39±2.37)pg·mL-1,respectively;the numbers of Bifidobacterium were 7.77±0.04,5.19±0.09 and 7.45±0.03,respectively;the numbers of Lactobacillus were 6.40±0.07,4.10±0.05 and 6.36±0.08,respectively.There were statistically significant differences in the above indexes between model group and control group,and between experimental group and model group(P＜0.01,P＜0.05).Conclusion Jichuan decoction can improve the defecation of rats with slow transit constipation.关键词
济川煎/慢传输型便秘/血管活性肠肽/P物质/肠道菌群Key words
Jichuan decoction/slow transit constipation/vasoactive intestinal peptide/substances P/intestinal flora分类
医药卫生引用本文复制引用
裴贵兵,张波,陈志成,姜华..济川煎对慢传输型便秘大鼠肠道功能的影响及作用机制的研究[J].中国临床药理学杂志,2024,40(8):1189-1193,5.基金项目
江苏省中医药局科技基金资助项目(YB2017099) （YB2017099）
江苏省老年健康科研基金资助项目(LX2021016) （LX2021016）
