中国临床药理学杂志2024，Vol.40Issue(8)：1194-1197,4.DOI:10.13699/j.cnki.1001-6821.2024.08.022
桔梗皂苷D对大鼠力竭运动性肾损伤的保护作用研究
Protective effect of platycodon platycodon D on renal injury induced by exhaustive exercise in rats
摘要
Abstract
Objective To analyze the protective effect of platycodon platycodon D on renal injury induced by exercise exhaustion in rats.Methods The model of kidney injury induced by exhaustive exercise was established by treadmill training.Fifty SD rats were divided into control group(0.9％NaCl),model group(exhaustive exercise model),experimental-L,-M,-H groups(25,50,100 mg·kg-1 platycodon D).Renal function[blood urea nitrogen(BUN),serum creatinine(Scr)],renal injury markers[neutrophil gelatinase associated lipocalin(NGAL),kidney injury molecule-1(KIM-1)]were detected by the kit;nucleotide oligomeric domain-like receptor family 3(NLRP3),apoptosis-associated speck-like protein containing a card(ASC),cysteine aspartic acid specific protease-1(Caspase-1)protein expressions were detected by Western blot.Results BUN levels in control group,model group,experimental-L,-M,-H groups were(5.52±0.49),(9.98±0.85),(8.30±0.65),(7.12±0.47)and(5.98±0.63)mmol·L-1;Scr were(1.69±0.24),(5.69±0.67),(4.71±0.47),(3.55±0.25)and(2.67±0.24)ng·mL-1;NGAL were(207.58±28.56),(613.42±63.87),(521.52±55.35),(419.89±40.85)and(278.13±26.48)pg·mL-1;KIM-1 were(12.11±1.52),(80.42±9.56),(69.03±7.58),(47.32±6.67)and(24.67±3.60)ng·L-1;NLRP3 protein expression were 0.43±0.06,1.35±0.11,1.03±0.10,0.78±0.09 and 0.53±0.06;ASC protein expression were 0.25±0.03,0.74±0.06,0.60±0.06,0.44±0.05 and 0.31±0.04;Caspase-1 protein expression were 0.31±0.04,0.83±0.07,0.69±0.06,0.53±0.05 and 0.42±0.04.Compared with the control group,the above indexes in the model group were statistically significant(all P＜0.01);compared with the model group,the above indexes of the experimental-L,-M,-H groups were statistically significant(all P＜0.01).Conclusion Platycodon D can inhibit the activation of NLRP3 inflammasome to alleviate the renal injury induced by exhaustive exercise in rats.关键词
桔梗皂苷D/力竭运动/肾功能/炎性反应/核苷酸寡聚化结构域样受体家族3炎性小体Key words
platycodon D/exhaustive exercise/kidney function/inflammatory response/nucleotide oligomeric domain-like receptor family 3 inflammasome分类
医药卫生引用本文复制引用
苏龙,陶瑞松..桔梗皂苷D对大鼠力竭运动性肾损伤的保护作用研究[J].中国临床药理学杂志,2024,40(8):1194-1197,4.基金项目
安徽省高校自然科学基金资助项目(KJ2020A0144) （KJ2020A0144）
