A Golden2-like transcription factor,BnGLK1a,improves chloroplast development,photosynthesis,and seed weight in rapeseedOACSTPCD
A Golden2-like transcription factor,BnGLK1a,improves chloroplast development,photosynthesis,and seed weight in rapeseed
Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and development.In this study,we demonstrate that Golden2-like 1a(BnGLK1a)plays an important role in regulating chloroplast development and photosynthetic efficiency.Overexpressing BnGLK1a resulted in significant increases in chlorophyll content,the number of thylakoid membrane layers and photosynthetic efficiency in Brassica napus,while knocking down BnGLK1a transcript levels through RNA interference(RNAi)had the opposite effects.A yeast two-hybrid screen revealed that BnGLK1a interacts with the abscisic acid receptor PYRABACTIN RESISTANCE 1-LIKE 1-2(BnPYL1-2)and CONSTITUTIVE PHOTOMORPHOGENIC 9 SIGNALOSOME 5A subunit(BnCSN5A),which play essential roles in regulating chloroplast development and photosynthesis.Consistent with this,BnGLK1a-RNAi lines of B.napus display hypersensitivity to the abscisic acid(ABA)response.Importantly,overexpression of BnGLK1a resulted in a 10％increase in thousand-seed weight,whereas seeds from BnGLK1a-RNAi lines were 16％lighter than wild type.We propose that BnGLK1a could be a potential target in breeding for improving rapeseed productivity.Our results not only provide insights into the mechanisms of BnGLK1a function,but also offer a potential approach for improving the productivity of Brassica species.
Qianwei Zhang;Yuxiang Jiang;Liezhao Liu;Kun Lu;Jiana Li;Yu Pan;Yuanyi Mao;Zikun Zhao;Xin Hu;Ran Hu;Nengwen Yin;Xue Sun;Fujun Sun;Si Chen
Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City,Southwest University,Chongqing 400715,China||Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China||Engineering Research Center of South Upland Agriculture,Ministry of Education,Chongqing 400715,ChinaAcademy of Agricultural Sciences,Southwest University,Chongqing 400715,China||Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education/College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China
Brassica napusBnGLK1achloroplast developmentphotosynthetic efficiencyyield
《农业科学学报（英文）》 2024 (005)
1481-1493 / 13
This work was funded by the National Natural Science Foundation of China(32172597 and 31830067),the Chongqing Talents of Exceptional Young Talents Project,China(CQYC202005097,cstc2021ycjh-bgzxm0204,and cstc2021jcyj-bshX0002),the China Agriculture Research System of MOF and MARA(CARS-12),the 111 Project,China(B12006),and the Germplasm Creation Special Program of Southwest University,China.The authors would like to thank Dr.Pu Yan(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,China)for providing the Nimble Cloning vectors and technical advice.
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