Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.OACSTPCD
Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.
Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2∶3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.
Xiaohui Wu;Yue Wu;Xiao Wang;Hongfei Liu;Wenzhao Dong;Xinyou Zhang;Mengyuan Zhang;Zheng Zheng;Ziqi Sun;Feiyan Qi;Hua Liu;Juan Wang;Mengmeng Wang;Ruifang Zhao
College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China||Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,ChinaHenan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,ChinaHenan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,China||The Shennong Laboratory,Zhengzhou 450002,ChinaShangqiu Academy of Agricultural and Forestry Sciences,Shangqiu 476000,China
peanut web blotchbulked segregant analysisKASP markersresistant gene
《农业科学学报（英文）》 2024 (005)
1494-1506 / 13
This work was supported by the Key Research Project of the Shennong Laboratory,China(SN01-2022-03),the Henan Provincial Science and Technology R&D Program Joint Fund(Superiority Discipline Cultivation)Project,China(222301420100),the Major Science and Technology Projects of Henan Province,China(221100110300),the China Agriculture Research System of MOF and MARA(CARS-13),the Henan Provincial Agriculture Research System,China(S2012-5),the Outstanding Young Scientists of Henan Academy of Agricultural Sciences,China(2022YQ16),and the Independent Innovation Project of the Henan Academy of Agricultural Sciences,China(2023ZC093).
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