中国骨伤2024，Vol.37Issue(4)：392-398,7.DOI:10.12200/j.issn.1003-0034.20221303
持续劳损加冰敷法兔冻结肩模型的制备及评价
Establishment and evaluation of a rabbit model of frozen shoulder induced by persistent strain injuries and ice com-pression
摘要
Abstract
Objective To evaluate the rabbit modle of frozen shoulder induced by persistent strain injuries and ice com-pression.Methods Twelve clean,healthy male New Zealand rabbits with a mass of(2 500±500)g were selected and randomly divided into a blank group and a control group with 6 rabbits in each group.In the control group,the rabbits were modeled with persistent strain injuries and ice compression,the general conditions of the rabbits and the active and passive activities of the shoulder joint were observed and their body weights were recorded.MRI was performed on the affected shoulder joints at 6 d and 29 d after modelling to observe the fluid and soft tissue;HE staining was used to observe the morphology of the rabbit bi-ceps longus tendon and the synovial membrane of the joint capsule;Masson staining was used to observe the fibrous deposits of the rabbit biceps longus tendon and the synovial membrane of the joint capsule,and the fibrous deposits were analysed semi-quantitatively by Image J software.Results Six days after the end of modeling,the active movement of the shoulder joints in the control group was limited,the passive movement was not significantly limited,and they walked with a limp;29 days after the end of the modeling,the active and passive movements of the shoulder joints in the model group were severely limited.Com-pared with the blank group(2.50±0.14)kg,the body weight of the model group(2.20±0.17)kg was significantly reduced(P＜0.01).MRI showed that 6 days after modelling,the muscles around the shoulder joint were not smooth in shape,the joint cap-sule structure was narrowed and a large amount of fluid was seen in the joint cavity;29 days after modelling,the muscles around the shoulder joint were rough in shape,structure of the joint capsule was unclear and the fluid in the joint cavity was reduced compared with 6 days after modelling.Pathological staining showed that the long-headed biceps tendon fibres in the control group were disorganised,curled or even broken,and the synovial tissue of the joint capsule was heavily vascularised,with col-lagen fibre deposits and severe inflammatory cell infiltration.The fiber deposition of the long head of biceps brachii in the mod-el group[(23.58±3.41)％,(27.56±3.70)％]and synovial tissue[(41.78±5.59)％,(62.19±7.54)％]were significantly higher than those in the blank group[(1.79±1.03)％,(1.29±0.63)％]at 7 and 30 days after modeling and synovial tissue fiber de-position[(8.15±3.61)％,(11.29±7.10)％],as shown by the semi-quantitative analysis of Masson staining results by Image J software.And the longer the time,the more severe the fibrosis(P＜0.01).Conclusion The behavioral,imaging and pathological findings showed that the rabbit frozen shoulder model with persistent strain injuries and ice compression is consistent with the clinical manifestations and pathogenesis of periarthritis,making it an ideal method for periarthritis research.关键词
冻结肩/模型/滑膜Key words
Frozen shoulder/Model/Synovial membrane分类
医药卫生引用本文复制引用
刘璐,程少丹,程杨,彭思琛,葛程,王世辉..持续劳损加冰敷法兔冻结肩模型的制备及评价[J].中国骨伤,2024,37(4):392-398,7.基金项目
上海市科委课题(编号:19401935000,20Y21901600) （编号:19401935000,20Y21901600）
上海市长宁区科委课题(编号:CNKW20185Y12) （编号:CNKW20185Y12）
上海市长宁区医学硕博士创新人才基地项目(编号:RCJD2022S03) （编号:RCJD2022S03）
上海市长宁区名中医建设项目(编号:CNMZY-2021013) （编号:CNMZY-2021013）
上海市长宁区青年中医医师培养项目(编号:2021CNQNZY002)Shanghai Municipal Science Commission Project(No.19401935000,20Y21901600) （编号:2021CNQNZY002）
