单细胞蛋白质成像方法进展
Progress of single-cell protein imaging methods
摘要
Abstract
The differential expression and subcellular localization of single-cell proteins are closely related to the physiological state and pathological mechanisms of the body.The development of single-cell protein in situ imaging methods provides powerful tools for spatial single-cell proteomics research and single-cell protein profiling.This article summarizes the single-cell protein imaging methods developed in recent years,including the circulating immunofluorescence imaging methods based on ordered multi-round antibody incubation,mass spectrometry imaging based on metal element labeled antibodies,fluorescence imaging based on DNA-barcoded antibody,gene encoded fluorescence protein imaging and spectral imaging based on Raman spectroscopy or X-ray spectroscopy,with brief explanation of the imaging principles of these methods.It focuses on the multiple performance,imaging resolution and signal amplification performance of these methods,and analyzes their application characteristics in practical scientific research and clinical work,in the hope of providing some reference for the development of more revolutionary single-cell imaging methods,and promoting the development of biomedical and precision medicine.关键词
单细胞蛋白质/免疫荧光/质谱成像/荧光蛋白/光谱成像/进展Key words
single-cell protein/immunofluorescence/mass spectrometry imaging/fluorescent protein/spectral imaging/progress分类
医药卫生引用本文复制引用
姚春露,张惟杰,张云龙,邓朝霞,王梦灵,张左玲,王琛,宋沁馨,邹秉杰..单细胞蛋白质成像方法进展[J].中国药科大学学报,2024,55(2):147-157,11.基金项目
国家自然科学基金项目(No.82173780) （No.82173780）
江苏省重点研发计划社会发展面上项目(No.BE2023843) （No.BE2023843）
药物质量与安全预警教育部重点实验室资助项目(No.DQCP20/21MS04) （No.DQCP20/21MS04）
江苏省研究生科研与实践创新计划项目(No.KYCX23-0854) （No.KYCX23-0854）
大学生创新创业训练计划项目(No.2023103161187) This study was supported by the National Natural Science Foundation Project(No.82173780) （No.2023103161187）
the Jiangsu Province Key R&D Program Social Development General Project(No.BE2023843) （No.BE2023843）
the Key Laboratory of Drug Quality and Safety Early Warning of the Ministry of Education(No.DQCP20/21MS04) （No.DQCP20/21MS04）
Jiangsu Province Graduate Research and Practice Innovation Program(No.KYCX23-0854) （No.KYCX23-0854）
and the Achievements of the College Student Innovation and Entrepreneurship Training Program Project(No.2023103161187) （No.2023103161187）
