中国药科大学学报2024，Vol.55Issue(2)：158-166,9.DOI:10.11665/j.issn.1000-5048.2023041303
邻近标记技术在膜蛋白相互作用中的研究进展
Progress of proximity labeling technology in membrane protein interaction
摘要
Abstract
Membrane proteins,which play a critical role in various life processes,particularly in regulating cell-cell contact and signal transduction,are closely linked to cell differentiation and maturation.Therefore,it is of great theoretical and practical significance to develop a variety of methods to thoroughly explore the interactions between membrane proteins.In addition to traditional techniques such as immunoprecipitation,newly developed proximity labeling(PL)techniques have gradually become important means to study membrane protein interaction.PL methods are based on engineered enzymes fused with bait protein to catalyze small molecules,label neighboring target proteins,and detect the interactions by flow cytometry,mass spectrometry,confocal microscopic imaging,etc.This paper focuses on the recent developments in PL techniques for studying membrane protein interactions,with a prospect of the potential future directions for research in this area.关键词
邻近标记技术/细胞膜蛋白/膜蛋白相互作用/工具酶Key words
proximity labeling/cell membrane protein/membrane protein interaction/engineered enzyme分类
生物科学引用本文复制引用
裴欣,李贵登,储卫华..邻近标记技术在膜蛋白相互作用中的研究进展[J].中国药科大学学报,2024,55(2):158-166,9.基金项目
This work was supported by the National Natural Science Foundation of China(No.81972875,No.32270994)and the Natural Science Foundation Outstanding Youth Fund of Jiangsu Province(No.BK20211505) 国家自然科学基金项目(No.32270994,No.81972875) （No.81972875,No.32270994）
江苏省杰出青年基金项目(No.BK2021505) （No.BK2021505）
