邻近标记技术在膜蛋白相互作用中的研究进展OA北大核心CSTPCD
Progress of proximity labeling technology in membrane protein interaction
膜蛋白是细胞多种生命活动的主要承担者,膜蛋白之间的相互作用参与调控细胞间的接触与信号传递,并与细胞的分化、成熟息息相关.因此,开发多种深入解析膜蛋白之间相互作用的方法具有重要的理论与现实意义.除了传统的免疫共沉淀方法之外,新开发的邻近标记技术已经逐渐成为了研究膜蛋白相互作用的重要手段.邻近标记技术主要利用生物工具酶标记与诱饵蛋白相互作用的邻近蛋白,并通过流式细胞术、质谱以及共聚焦显微成像技术等方法检测膜蛋白之间的相互作用.本文着重介绍了近些年来新开发的用于研究膜蛋白相互作用的多种邻近标记技术,并展望了该类技术在膜蛋白相互作用领域未来的发展前景.
Membrane proteins,which play a critical role in various life processes,particularly in regulating cell-cell contact and signal transduction,are closely linked to cell differentiation and maturation.Therefore,it is of great theoretical and practical significance to develop a variety of methods to thoroughly explore the interactions between membrane proteins.In addition to traditional techniques such as immunoprecipitation,newly developed proximity labeling(PL)techniques have gradually become important means to study membrane protein interaction.PL methods are based on engineered enzymes fused with bait protein to catalyze small molecules,label neighboring target proteins,and detect the interactions by flow cytometry,mass spectrometry,confocal microscopic imaging,etc.This paper focuses on the recent developments in PL techniques for studying membrane protein interactions,with a prospect of the potential future directions for research in this area.
裴欣;李贵登;储卫华
中国药科大学生命科学与技术学院,南京 211198||中国医学科学院苏州系统医学研究所,苏州 215123中国医学科学院苏州系统医学研究所,苏州 215123中国药科大学生命科学与技术学院,南京 211198
生物学
邻近标记技术细胞膜蛋白膜蛋白相互作用工具酶
proximity labelingcell membrane proteinmembrane protein interactionengineered enzyme
《中国药科大学学报》 2024 (002)
158-166 / 9
This work was supported by the National Natural Science Foundation of China(No.81972875,No.32270994)and the Natural Science Foundation Outstanding Youth Fund of Jiangsu Province(No.BK20211505) 国家自然科学基金项目(No.32270994,No.81972875);江苏省杰出青年基金项目(No.BK2021505)
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