中国农业科技导报2024，Vol.26Issue(5)：129-137,9.DOI:10.13304/j.nykjdb.2023.0159
菌糠水提液对马铃薯致病疫霉的抑制机理
Inhibition Mechanism of Water Extract from Spent Mushroom Substrate against Phytophthora infestans in potatoes
摘要
Abstract
Mushroom substrate is the residue of mushroom production,which is rich in inorganic salts and organic matter.The water extract from spent mushroom substrate(WESMS)of Lentinula edodes was prepared by hot water extraction,the ultraviolet(UV)absorption,surface charge and particle size distribution were characterized using an UV spectrophotometer,Zeta potential and particle size analyzer.The inhibition rate of WESMS against Phytophthora infestans was calculated by plate penetration and cross diameter measurement.Further,both optical microscopy and scanning electron microscopy were used to observe the damage caused by WESMS to the morphology of the pathogen cells.Subsequently,the impact of WESMS on the DNA of Phytophthora infestans was analyzed by agarose gel electrophoresis.The results showed that WESMS could absorb UV rays at A(UVA,315～400 nm),B(UVB,280～315 nm)and C(UVC,100～280 nm)bands.Moreover,the particle size distribution in water was 3 649.27 nm.The antibacterial experiment showed that WESMS had an inhibitory effect on the growth of pathogens.The inhibitory effect became more obvious with the increase of the volume percentage concentration of WESMS.The inhibition rate of WESMS against pathogens was nearly 100%at a high volume concentration of 6.25%.The mycelia of pathogenic cells treated with WESMS exhibited obvious distortion,flatness and wrinkles compared with the control group,which indicated that WESMS could cause severe damage to cells.In addition,the DNA band brightness of the treatment group was darker than that of the control group,and the degree of DNA damage was positively related to the volume percentage concentration of WESMS.Above results provided scientific basis and technical support for the utilization of spent mushroom substrate and its use in the effective control of potato late blight.关键词
香菇菌糠/马铃薯晚疫病/致病疫霉/抗菌机理Key words
spent mushroom substrate of Lentinula edodes/potato late blight/Phytophthora infestans/antimicrobial mechanism分类
农业科技引用本文复制引用
莫雯婧,陈洪森,桂芳泽,洪慈清,蔡鑫铠,关雄,潘晓鸿..菌糠水提液对马铃薯致病疫霉的抑制机理[J].中国农业科技导报,2024,26(5):129-137,9.基金项目
国家重点研发计划项目(2022YFD1400700) （2022YFD1400700）
福建农林大学茶树绿色栽培与生态茶园建设研究项目(K1520007A03) （K1520007A03）
福建农林大学科技发展资金项目(KFB23012). （KFB23012）
