中国农业科技导报2024，Vol.26Issue(5)：138-147,10.DOI:10.13304/j.nykjdb.2023.0281
樟叶总木脂素提取工艺及其抑菌活性研究
Study on Extraction Process and Antibacterial Activity of Lignans from Cinnamomum camphora Leaves
摘要
Abstract
To study the optimum reflux extraction process and antifungal activity of total lignans from Cinnamomum camphora leaves,the leaves of C.camphora were as material,and the contents of sesamin and 9-hydroxysesamin in C.camphora leaves were determined.The reflux extraction process of total lignans from C.camphora was optimized by single factor combined with Box-Behnken response surface method,and the antifungal activity of total lignans was evaluated by toxic plate method.The results showed that the optimum conditions of reflux extraction were as follows:extraction temperature 86℃,ratio of solid to liquid 1∶8.39(g·mL-1),extraction time 43 min and anhydrous ethanol as extraction solvent.Under these conditions,the extraction rate of lignans was 0.380 4%.The influence of factors on the extraction rate was extraction temperature>extraction time>solid-liquid ratio.The antifungal activity test showed that the antifungal effects of lignans on Gliocladium catenulatum,Valsa mali Miyabe et Yamada,Fusarium oxysporumf and Pythium aphanidermatum were the best with 2 mg·mL-1,and the antifungal rates were more than 50%.The EC50 values were 1.238,1.280,1.486 and 0.419 mg·mL-1,respectively.Above results provided theoretical references for the subsequent development and utilization of C.camphora leaves and the research and development of plant-derived pesticides.关键词
樟/木脂素/响应面法/抑菌活性/毒力Key words
Cinnamomum camphora/lignan/response surface method/antibacterial activity/toxicity分类
农业科技引用本文复制引用
张岚雄,郑威,陈源桉,沈婧,邹双全,伍建榕,倪林..樟叶总木脂素提取工艺及其抑菌活性研究[J].中国农业科技导报,2024,26(5):138-147,10.基金项目
福建省林业科技研究项目(No.2023FKJ28) （No.2023FKJ28）
福建省科技计划项目(2023N0003). （2023N0003）
