中国农业科技导报2024，Vol.26Issue(5)：148-155,8.DOI:10.13304/j.nykjdb.2024.0144
娄彻氏链霉菌HM85的鉴定及其对甜菜的防病促生作用
Identification of Streptomyces rochei HM85 and Its Biocontrol and Growth-promoting Effects on Sugar Beet
摘要
Abstract
Cercospora leaf sport of beet(CLS),caused by Cercospora beticola,is the most destructive foliar fungal disease of sugar beet worldwide which seriously restricts the development of China's sugar industry.In order to screen and identify the antagonistic strains with biocontrol applications against Cercospora beticola,the rhizosphere soil samples of sugar beet were collected from Hulunbuir,Inner Mongolia autonomous region and the antagonistic strain HM85 with stable antimicrobial activity was screened by dilution coating flat plate method and dual culture assay.The strain HM85 was identified as Streptomyces rochei based on its morphological,physiological and biochemical characteristics along with 16S rRNA gene sequence analysis.The results of dual culture test showed that the HM85 strain could significantly inhibit the growth of Cercospora beticola HB2-2-2 with inhibitory rate of 58.68%.In addition,the fermentation broth of S.rochei HM85 had antagonistic activity against Cercospora beticola and could promote the growth of sugar beet in pot tests,which plant height,fresh weight of plant,root length,fresh weight of root and root diameter was increased by 22.73%,20.47%,36.10%,32.52%,75.03%compared to the control,respectively.In summary,S.rochei HM85 could be exploited as a potential bio-inoculant improving production and biocontrol agent for sugar beet leaf sport disease control.关键词
娄彻氏链霉菌/甜菜尾孢菌/甜菜褐斑病/拮抗活性/促生作用Key words
Streptomyces rochei/Cercospora beticola/Cercospora leaf sport of beet(CLS)/antagonistic activity/growth-promoting effect分类
农业科技引用本文复制引用
杨智敏,程晓宁,随洋,王瑞利,于超,赵玲玲,陈春梅,雅茹,贾丽,张明月,王宏伟,张慧豪,姚淞耀,赵莹,邵科,张园园,杜红岩,刘晓东,侯亚光,王毅,徐道龙,黄金贵..娄彻氏链霉菌HM85的鉴定及其对甜菜的防病促生作用[J].中国农业科技导报,2024,26(5):148-155,8.基金项目
内蒙古自治区科技计划项目(2020GG0271) （2020GG0271）
国家糖料产业技术体系建设项目(CARS-170725) （CARS-170725）
内蒙古自治区"揭榜挂帅"项目(2022JBGS0029) （2022JBGS0029）
内蒙古自治区科技成果转化专项资金项目(2021CG0042). （2021CG0042）
