中国农业科技导报2024，Vol.26Issue(5)：156-166,11.DOI:10.13304/j.nykjdb.2023.0878
抗菌肽PAJE对金黄色葡萄球菌及其SCVs的杀菌作用机制研究
Bactericidal Mechanism of Antimicrobial Peptide PAJE Against Staphylococcus aureus and SCVs
摘要
Abstract
Staphylococcus aureus(S.aureus)and the small colony variants(SCVs)infection are considered as main issues affect livestock and poultry health.However,there are still many challenges in persistent and recurrent infections,and even drug resistance for treatment of traditional antibiotics.The antimicrobial peptide PAJE has the broad-spectrum antibacterial activity.To study the mechanisms of peptide PAJE against S.aureus and SCVs,the antibacterial activity,stability and biological characteristics were determined.The results indicated that the antibacterial peptide PAJE had a penetration ability to destruct bacterial cell wall,even permeated 54.7%and 62.9%cell membrane.Additionally,the peptide PAJE could also up-regulate the Agr regulatory system genes,down-regulate the SigB regulatory system and biofilm forming related genes,which greatly inhibited biofilm formation.In addition,higher temperature and pH stability made PAJE better processing,storage,and environmental tolerance.In conclusion,the antimicrobial peptide PAJE could be a potential candidate peptide for the treatment of S.aureus,SCVs and their biofilm infections,which provided theoretical support for the development of reduce and replace antibiotics products.关键词
金黄色葡萄球菌/小菌落突变体/抗菌肽PAJE/生物膜Key words
Staphylococcus aureus/SCVs/antimicrobial peptide PAJE/biofilm分类
农业科技引用本文复制引用
高思琦,杨新建,朱德琪,关明玮,寇云婷,满程,焦健..抗菌肽PAJE对金黄色葡萄球菌及其SCVs的杀菌作用机制研究[J].中国农业科技导报,2024,26(5):156-166,11.基金项目
北京城市学院种子基金项目(KYZZ202005). （KYZZ202005）
