中国麻业科学2024，Vol.46Issue(2)：80-89,10.
用于工业大麻种质资源筛选和鉴定的KASP标记集开发
Development of KASP Marker Set for Screening and Identification of Industrial Hemp Germplasm Resources
摘要
Abstract
Currently,the molecular screening and identification of industrial hemp germplasm re-sources in China mainly rely on methods such as AFLP,SSR and Resequencing,lacking in a fast,accu-rate,and intuitive molecular marker system.The development of Kompetitive Allele Specific PCR(KASP)with marker sets and its detection system has good application prospects.This study first suc-cessfully developed a specific KASP locus based on the cannabinoid B locus gene polymorphism,which can accurately distinguish different chemical types of low-THC,high-THC or intermediate types in can-nabis germplasm resources.A total of 32 high-quality core KASP markers were developed based on the whole-genome resequencing single nucleotide polymorphism(SNP)data from 95 different cannabis germ-plasm resources.Verification experiment showed that the 33 KASP marker sets and its detection system can be applied to industrial hemp assisted breeding,molecular identification of industrial hemp varieties,and fingerprint library construction,enriching the types and methods of industrial hemp molecular mark-ers.关键词
工业大麻/种质鉴定/大麻素/KASP标记/指纹图谱Key words
industrial hemp/germplasm identification/cannabinoid/KASP marker/fingerprint分类
农业科技引用本文复制引用
周佳,陈璇,张园,郭孟璧,吕品,杜光辉,许艳萍,杨明,张庆滢,字雪靖..用于工业大麻种质资源筛选和鉴定的KASP标记集开发[J].中国麻业科学,2024,46(2):80-89,10.基金项目
财政部和农业农村部:国家麻类产业技术体系育种技术与方法岗位(CARS-16-E02) （CARS-16-E02）
云南省科技计划项目(202305AP350011) （202305AP350011）
云南省高层次人才培养支持计划(青年拔尖) （青年拔尖）
