中国药房2024，Vol.35Issue(9)：1070-1075,6.DOI:10.6039/j.issn.1001-0408.2024.09.08
基于蛋白质组学探讨丹参饮对高脂血症模型大鼠血脂异常的调节机制
Exploration of the regulatory mechanism of Danshen decoction on dyslipidemia in hyperlipidemia model rats based on proteomics
摘要
Abstract
OBJECTIVE To explore the regulatory mechanism of Danshen decoction on dyslipidemia in hyperlipidemia model rats.METHODS The experimental rats were divided into blank group(n=9,no modeling),model group(n=8,modeling),and Danshen decoction group(n=9,modeling).Starting from the 9th week of feeding with the high-fat diet,rats in the Danshen decoction group were given the corresponding medication solution(3.6 g/kg)intragastrically,while blank group and model group were given equal volume of normal saline,once a day,for 4 consecutive weeks.After 4 weeks of administration,the plasma levels of triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were measured in each group of rats;the pathological and morphological changes of liver tissue were observed;the differential proteins between samples were screened out by TMT quantitative proteomic analysis;the expression levels of the key differentially expressed proteins in the liver,including epoxide hydrolase 2(EPHX2),perilipin 2(PLIN2),peroxisome proliferator activated receptor γ(PPAR γ)and glycogen synthase kinase-3β(GSK-3β)were detected.RESULTS Compared with the model group,the plasma levels of TC,TG and LDL-C in the Danshen decoction group were significantly reduced(P＜0.05),while the level of HDL-C was significantly increased(P＜0.05).The liver tissue of rats in model group showed uneven staining,disordered arrangement of liver plates,disappearance of liver sinusoids,nuclear condensation or disappearance of some cells,swelling and fusion of cytoplasm,proliferation of connective tissue,and diffuse vacuolar-like fat droplet changes.The liver tissue of Danshen decoction group showed varying degrees of improvement in the above pathological and morphological.The results of differential protein analysis showed that the total number of differential proteins was 298 between the model group and the blank group;the total number of differential proteins was 139 between the model group and Danshen decoction group.Compared with the model group,the expression levels of EPHX2 and PLIN2 proteins in the liver tissue of rats in the Danshen decoction group were significantly reduced(P＜0.01),while the expression levels of GSK-3β and PPARγ were significantly increased(P＜0.01).CONCLUSIONS Danshen decoction has a significant improvement effect on the plasma lipid levels and the pathological and morphological of the liver tissue in hyperlipidemia model rats.Its regulatory mechanism may be related to the up-regulation of PPARγ and GSK-3β expression and down-regulation of EPHX2 and PLIN2 expression,and the signaling pathways involved may include PPAR-γ signal pathway.关键词
高脂血症/丹参饮/蛋白质组学/差异蛋白/过氧化物酶体增殖物激活受体γ/糖原合酶激酶3βKey words
hyperlipidemia/Danshen decoction/proteomics/differential protein/peroxisome proliferator activated receptor γ/glycogen synthase kinase-3β分类
医药卫生引用本文复制引用
张玉昆,冯月男,卞敬琦,刘欣欣,肖洪彬,牛雯颖..基于蛋白质组学探讨丹参饮对高脂血症模型大鼠血脂异常的调节机制[J].中国药房,2024,35(9):1070-1075,6.基金项目
国家自然科学基金面上项目(No.82074326,No.82274405) （No.82074326,No.82274405）
黑龙江省中医药管理局中医药科研项目(No.ZHY2020-084) （No.ZHY2020-084）
国家中医药考试2022年度科研课题(No.TC2022021) （No.TC2022021）
黑龙江省博士后资助项目(No.LBH-Z20195) （No.LBH-Z20195）
