中国畜牧杂志2024，Vol.60Issue(5)：206-212,7.DOI:10.19556/j.0258-7033.20230731-02
HSD17B12对兔卵巢颗粒细胞增殖和凋亡的影响
Effect of HSD17B12 on Proliferation and Apoptosis of Granulosa Cells in Rabbit Ovary
摘要
Abstract
This study aimed to study the effect of hydroxysteroid 17-β dehydrogenase 12(17 β hydroxysteroid dehydrogenase type 12,HSD17B12)on the proliferation and apoptosis of granulosa cells in rabbit ovary.Rabbit HSD17B12 gene was cloned and bioinformatics analysis;primary rabbit granule cells(Granulosa cells,GCs)were isolated and identified by FSHR immunofluorescence;HSD17B12 gene overexpression plasmid and interfering RNA were constructed,transfected with GCs,the effect of HSD17B12 on proliferation and apoptotic gene expression was detected by qRT-PCR,and the effect of HSD17B12 on GCs proliferation and apoptosis was detected by cell flow and CCK 8.The results showed that:1 The isolated primary cells were identified as GCs and were positive for FSHR expression using FSHR immunofluorescence staining.2 cloned the HSD17B12 gene and found its cDNA of 938 bp encoding 312 amino acids.What about homology?3.Overexpression of HSD17B12 can significantly increase the expression of proliferation-related genes AKT 1-1(P<0.01),significantly reduce the expression of apoptosis-related genes Bad,Bad,Fas,FasL,P53(P<0.01),and significantly reduce the expression of Bcl-2(P<0.05);however,after interference.4.HSD17B12 can significantly promote the proliferation of GCs and inhibit the apoptosis of GCs(P<0.01).The conclusion is HSD17B12 may regulate the expression of AKT 1,Bad,Fas,FasL,P53 and other genes to significantly promote the proliferation of rabbit GCs and inhibit their apoptosis,and provide a theoretical basis for the mechanism of its role in the ovarian development of female rabbits.关键词
HSD17B12/兔/颗粒细胞/增殖/凋亡Key words
HSD17B12/Rabbit/Granulosa cells/Proliferation/Apoptosis分类
农业科技引用本文复制引用
韩旭东,任宇尧,刘燕,赵博昊,吴信生,陈阳..HSD17B12对兔卵巢颗粒细胞增殖和凋亡的影响[J].中国畜牧杂志,2024,60(5):206-212,7.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-43-A-1) （CARS-43-A-1）
江苏现代农业产业技术体系建设专项(JATS[2023]445) （JATS[2023]445）
浙江省农业新品种选育重大科技专项(2021C02068-7) （2021C02068-7）
扬州大学青蓝工程项目 （）
