中国药房2024，Vol.35Issue(9)：1082-1086,5.DOI:10.6039/j.issn.1001-0408.2024.09.10
清上蠲痛汤标准汤剂挥发油的UPLC特征图谱建立及成分分析
Establishment of UPLC characteristic chromatogram and component analysis of the volatile oil in the standard decoction of Qingshang juantong decoction
摘要
Abstract
OBJECTIVE To establish the characteristic chromatogram of the volatile oil in the standard decoction of Qingshang juantong decoction,and preliminarily infer the main active components of volatile oil that affect the clinical therapeutic effect.METHODS The volatile oil in the standard decoction of Qingshang juantong decoction was extracted by steam distillation.The ultra-high performance liquid chromatography(UPLC)characteristic chromatograms of 15 batches of samples were established by the Similarity Evaluation System of TCM Chromatographic Fingerprint(2012 edition),and the similarity evaluation was carried out.The volatile oil of standard decoction was identified by UPLC coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS).Then the volatile oil components were analyzed by GC-MS.RESULTS The similarities of UPLC characteristic chromatograms for volatile oil of 15 batches of Qingshang juantong decoction were between 0.949 and 0.997.A total of 12 common peaks were obtained.According to the UPLC-Q-TOF/MS,the main components were methyl eugenol,E-ligustilide,E-butylidenephthalide and so on.A total of 23 components were identified by GC-MS,which were mainly 3,4,5-trimethoxy-methylbenzene,patchouli alcohol,Z-ligustilide and so on.CONCLUSIONS The characteristic chromatograms of the volatile oil in the standard decoction of Qingshang juantong decoction is established,and it is inferred that methyl eugenol,ligustilide,E-butylidenephthalide,patchouli alcohol,3,4,5-trimethoxy-methylbenzene might be the main active components affecting the clinical therapeutic effect of the volatile oil of Qingshang juantong decoction.关键词
清上蠲痛汤/经典名方/挥发油/特征图谱/化学成分Key words
Qingshang juantong decoction/classic formula/volatile oil/characteristic chromatogram/chemical constituents分类
医药卫生引用本文复制引用
范志颖,李松,祝倩倩,王协和,翟燕娟,王慧敏,顾杨欣,周海琴,陆兔林,张科卫..清上蠲痛汤标准汤剂挥发油的UPLC特征图谱建立及成分分析[J].中国药房,2024,35(9):1082-1086,5.基金项目
江苏省科技成果转化专项资金项目(No.BA2021018) （No.BA2021018）
