中国药房2024，Vol.35Issue(9)：1100-1107,8.DOI:10.6039/j.issn.1001-0408.2024.09.13
TMZ@ZIF-8纳米粒的制备、表征及体内外抗肿瘤研究
TMZ@ZIF-8 nanoparticles:preparation,characterization,and in vivo and in vitro anti-tumor studies
摘要
Abstract
OBJECTIVE To prepare zeolite imidazole framework(ZIF)-8 nanoparticles(NPs)loaded with temozolomide(TMZ)(abbreviated as TMZ@ZIF-8 NPs)drug delivery system,thus increasing drug enrichment and anti-glioma effects in lesions.METHODS After preparing ZIF-8 NPs using the room temperature solution reaction method,the impregnation method was used to prepare TMZ@ZIF-8 NPs drug delivery system.Characterization was carried out using transmission electron microscopy,laser particle size,and Fourier transform infrared spectroscopy,and dissolution and anti-tumor activity experiments in vitro and in vivo were conducted.RESULTS TMZ@ZIF-8 NPs were successfully prepared with the particle size of(126.23±7.92)nm,drug loading amount of(28.79±1.26)%,and 72 h cumulative dissolution rate of(72.36±3.62)%.The results of in vitro anti-tumor activity experiments showed that the relative cell survival rate of ZIF-8 NPs remained above 90%;the prepared TMZ@ZIF-8 NPs delivery system exhibited superior inhibition,higher uptake capacity,and better promoting apoptosis effects on the growth and proliferation of C6 cells as compared with the free TMZ.The results of in vivo anti-tumor activity experiments showed that ZIF-8 NPs were not enriched in the brain of rats,and the enrichment effect of TMZ in the brain was not significant,while TMZ@ZIF-8 NPs had a significant enrichment effect in the brain.CONCLUSIONS ZIF-8 NPs can effectively load TMZ,and successfully prepared TMZ@ZIF-8 NPs can improve TMZ uptake ability and anti-glioma effect.关键词
替莫唑胺/沸石咪唑骨架/pH敏感/脑胶质瘤/药物递送系统/纳米粒Key words
temozolomide/zeolite imidazole framework/pH-sensitive/glioma/drug delivery system/nanoparticle分类
化学化工引用本文复制引用
刘婷,王怡,李冰辉,何硕,夏江,朱婷..TMZ@ZIF-8纳米粒的制备、表征及体内外抗肿瘤研究[J].中国药房,2024,35(9):1100-1107,8.基金项目
黑龙江省中医药管理局中医药科研课题(No.ZHY2023-211,No.ZHY2023-210) （No.ZHY2023-211,No.ZHY2023-210）
2023年度哈尔滨商业大学"青年科研创新人才"培育计划(No.2023-KYYWF-1028) （No.2023-KYYWF-1028）
哈尔滨商业大学博士后科研启动项目(No.BS0087) （No.BS0087）
哈尔滨商业大学博士科研支持计划(No.22BQ46,No.22BQ57) （No.22BQ46,No.22BQ57）
