中国医药科学2024，Vol.14Issue(7)：12-15,4.DOI:10.20116/j.issn2095-0616.2024.07.03
HPLC法测定蒙药沙蓬茎、叶、花中槲皮素和异鼠李素的含量
Determination of quercetin and isorhamnetin in the stems,leaves,and flowers of Mongolian medicine Agriophyllum squarrosum by HPLC
摘要
Abstract
Objective To determine the content of quercetin and isorhamnetin in the stems,leaves,and flowers of Mongolian medicine Agriophyllum squarrosum,and analyze the distribution of quercetin and isorhamnetin in different parts of Agriophyllum squarrosum.Methods High-performance liquid chromatography(HPLC)was used to determine the content of quercetin and isorhamnetin.Results Quercetin and isorhamnetin had a good linear relationship with the peak area within the range from 0.0107 to 0.5336 μg,0.0421 to 2.1038 μg,respectively,with recovery rates of 100.06%and 102.59%,and relative standard deviation(RSD)of 1.52%and 1.67%,respectively(n=6).The total content of quercetin and isorhamnetin in the stems,leaves,and flowers of Agriophyllum squarrosum is in the order of leaves>flowers>stems.Conclusion This method has high precision and good separation,and can be used for content determination of quercetin and isorhamnetin in the stems,leaves,and flowers of Mongolian medicine Agriophyllum squarrosum.关键词
蒙药/沙蓬/茎、叶、花/异鼠李素/槲皮素/高效液相色谱法Key words
Mongolian medicine/Agriophyllum squarrosum/Stems,leaves,and flowers/Isorhamnetin/Quercetin/High-performance liquid chromatography分类
医药卫生引用本文复制引用
乌雅汉,莫日根,呼和,额尔敦呼,乌日汗,白明君,包书茵,包桂花..HPLC法测定蒙药沙蓬茎、叶、花中槲皮素和异鼠李素的含量[J].中国医药科学,2024,14(7):12-15,4.基金项目
国家自然科学基金(82004070) （82004070）
内蒙古自治区"高等学校青年科技人才发展项目"(NJYT23134) （NJYT23134）
内蒙古自治区"草原英才"工程青年创新人才 （）
内蒙古自治区科技计划项目(2020GG0103) （2020GG0103）
内蒙古自治区蒙医药标准化项目(2023-MB030) （2023-MB030）
内蒙古自治区自然科学基金项目(2023LHMS08047) （2023LHMS08047）
2019内蒙古自治区科技重大专项项目(2019ZD004) （2019ZD004）
