中国中医药信息杂志2024，Vol.31Issue(5)：55-60,6.DOI:10.19879/j.cnki.1005-5304.202310042
基于Nrf2/HO-1通路探讨大承气汤治疗小鼠重症急性胰腺炎并发急性肾损伤作用机制
Exploration on the Mechanism of Dachengqi Decoction in Treating Severe Acute Pancreatitis Complicated with Acute Kidney Injury in Mice Based on Nrf2/HO-1 Pathway
摘要
Abstract
Objective To investigate the possible mechanism of Dachengqi Decoction in the treatment of severe acute pancreatitis(SAP)complicated with acute kidney injury(AKI).Methods SAP mouse model was prepared by retrograde injection of 3.5%sodium taurocholate into bile pancreatic duct.Thirty C57BL/6 mice were divided into sham-operation group,model group and the Dachengqi Decoction group.Dachengqi Decoction group was given Dachengqi Decoction for gavage at 5,13 and 21 hours after operation,the sham-operation group and the model group were given normal saline.The general condition of mice was observed 24 h after operation.The levels of AMY,TNF-α,IL-6,IL-1β,SCr,BUN,SOD and MDA in serum were detected.The morphology of pancreas and kidney was observed,and the mRNA and protein expressions of Nrf2 and HO-1 in renal tissue were detected.Results Compared with the sham operation group,the mice in the model group were sluggish and had poor response,and the contents of serum AMY,TNF-α,IL-1β,IL-6,SCr,BUN and MDA were significantly increased(P<0.001),while the content of SOD was significantly decreased(P<0.001);cell edema,necrosis and inflammatory cells infiltration were observed in pancreas and renal tissue,and the pathological scores were significantly increased(P<0.001),and the expression of Nrf2 mRNA and protein in renal tissue were significantly increased(P<0.01,P<0.05),and HO-1 protein expression was significantly increased(P<0.01).Compared with the model group,the overall condition of the mice in the Dachengqi decoction group was acceptable,the contents of serum AMY,TNF-α,IL-1β,IL-6,SCr,BUN and MDA in mice were significantly decreased(P<0.001,P<0.01),the contents of SOD increased significantly(P<0.05);the pancreas and renal tissue injuries were alleviated,the pathological scores were significantly decreased(P<0.05,P<0.001),the expressions of Nrf2 and HO-1 mRNA and protein in renal tissue significantly increased(P<0.05,P<0.01).Conclusion Dachengqi Decoction can alleviate the systemic inflammatory response and improve the pathological damage of pancreas and renal tissue in mice with SAP,and its mechanism may related to activate Nrf2/HO-1 signaling pathway and regulate oxidative stress response.关键词
重症急性胰腺炎/急性肾损伤/大承气汤/Nrf2/HO-1通路/小鼠Key words
severe acute pancreatitis/acute kidney injury/Dachengqi Decoction/Nrf2/HO-1 pathway/mice分类
医药卫生引用本文复制引用
吴月,王启蒙,孔婧,刘宇洋,宇萌,王晓素,李英..基于Nrf2/HO-1通路探讨大承气汤治疗小鼠重症急性胰腺炎并发急性肾损伤作用机制[J].中国中医药信息杂志,2024,31(5):55-60,6.基金项目
上海市卫生健康委员会中医药科研项目(2020JP022) （2020JP022）
上海市申康医院发展中心中西医临床协作试点建设项目(ZY2018-2020-FWTX-1004) （ZY2018-2020-FWTX-1004）
国家自然科学基金(82004264) （82004264）
