中国中医药信息杂志2024，Vol.31Issue(5)：92-98,7.DOI:10.19879/j.cnki.1005-5304.202309338
越婢汤对阿霉素诱导的人脐静脉内皮细胞高通透性的影响及机制研究
Study on the Effects and Mechanism of Yuebi Decoction on Adriamycin-induced Hyperpermeability of Human Umbilical Vein Endothelial Cells
摘要
Abstract
Objective To investigate the effects of Yuebi Decoction on adriamycin-induced hyperpermeability of human umbilical vein vascular endothelial cells(HUVEC);To explore the possible mechanism of its therapeutic effect on edema in nephrotic syndrome.Methods HUVEC were divided into control group,model group and Yuebi Decoction low-,medium-,and high-dosage groups(200,300,400 μg/mL).CCK-8 method was used to detect cell viability,Transwell method was used to detect cell permeability,Western blot was used to detect the expression of apoptosis signal regulated kinase 1(ASK1),zonula occludens-1(ZO-1),vascular endothelial-cadherin(VE-cadherin),and p38 protein,immunofluorescence method was used to detect the expression of ZO-1 and VE-cadherin.Results Compared with the control group,the cell activity of HUVEC in the model group was significantly decreased,cell permeability significantly increased,and cell swelling and membrane structure damage occurred,the expressions of ASK1 and p-p38 protein significantly increased,while the expressions of ZO-1 and VE-cadherin protein were significantly decreased(P<0.01).Compared with the model group,the cell activity and permeability of Yuebi Decoction low-,medium-,and high-dosage groups significantly increased,and the expressions of ASK1 and p-p38 protein were significantly decreased,and the expressions of ZO-1 and VE-cadherin protein significantly increased(P<0.05,P<0.01).Conclusion Yuebi Decoction can improve the high permeability of HUVEC induced by adriamycin,and its mechanism may be related to the inhibition of ASK1/p38 signaling pathway and upregulation of cell junction protein expression.关键词
越婢汤/人脐静脉血管内皮细胞/肾病综合征/凋亡信号调节激酶1/p38Key words
Yuebi Decoction/HUVEC/nephrotic syndrome/ASK1/p38分类
医药卫生引用本文复制引用
程苏,李婷婷,王艳茹,邵命海..越婢汤对阿霉素诱导的人脐静脉内皮细胞高通透性的影响及机制研究[J].中国中医药信息杂志,2024,31(5):92-98,7.基金项目
国家自然科学基金面上项目(81973807) （81973807）
