中国中医药信息杂志2024，Vol.31Issue(5)：112-117,6.DOI:10.19879/j.cnki.1005-5304.202310145
基于HPLC指纹图谱及色度值的当归与油当归比较研究
Comparative Study on the Difference between Angelicae Sinensis Radix and Oil Angelicae Sinensis Radix Based on HPLC Fingerprint and Chromaticity Value
摘要
Abstract
Objective To analyze the difference between Angelicae Sinensis Radix and oil Angelicae Sinensis Radix by establishing a fingerprint and combining the chromaticity value.Methods HPLC method was used to establish the fingerprint before and after frying Angelicae Sinensis Radix with oil,and the similarity was evaluated and the common peak was identified.Taking the total peak area and chromaticity value before and after frying Angelicae Sinensis Radix with oil as the indicators,paired sample t-test,cluster heat map analysis,principal component analysis and orthogonal partial least squares-discriminant analysis(OPLS-DA)were used to distinguish before and after frying Angelicae Sinensis Radix with oil.The differential markers were screened out by variable importance projection(VIP)>1.Results The established fingerprints of Angelicae Sinensis Radix and oil Angelicae Sinensis Radix were calibrated with 16 and 18 common peaks,respectively,among which,chromatographic peaks 8 and 10 were generated after processing.A total of 6 components were identified,including ferulic acid,chlorogenic acid,ligustilide,senkyunolide I,senkyunolide H,and ferulic acid pine ester.The clustering heatmap and principal component analysis results were consistent with the OPLS-DA results,and the samples could be clearly clustered into two categories:Angelicae Sinensis Radix and oil Angelicae Sinensis Radix.Through VIP value screening,peaks 5,7(chlorogenic acid),17,4,18(ligustilide),9(ferulic acid),1,13,11(senkyunolide I),2,and 3 were identified as the main chromatographic peaks that caused sample differences.The difference in chromaticity value ΔE* between Angelicae Sinensis Radix and oil Angelicae Sinensis Radix was 6.10 to 12.37,indicating that they could be recognized by the naked eye,and L*,a* and b* could all be used as key indicators for distinguishing Angelicae Sinensis Radix from oil Angelicae Sinensis Radix.Conclusion The established HPLC fingerprinting method for Angelicae Sinensis Radix and oil Angelicae Sinensis Radix is stable and reliable,and can be used to distinguish Angelicae Sinensis Radix from oil Angelicae Sinensis Radix by combining the difference in chromaticity value.关键词
当归/油当归/指纹图谱/色度值/多元统计分析Key words
Angelicae Sinensis Radix/oil Angelicae Sinensis Radix/fingerprint/chromaticity value/multivariate statistical analysis分类
医药卫生引用本文复制引用
党文飞,张红伟,周洁,周晶晶,窦霞,靳子明..基于HPLC指纹图谱及色度值的当归与油当归比较研究[J].中国中医药信息杂志,2024,31(5):112-117,6.基金项目
甘肃省中药炮制技术传承基地项目(2022年) （2022年）
甘肃省自然科学基金(21JR7RA574) （21JR7RA574）
甘肃省中医药科研课题(GZKP-2022-23) （GZKP-2022-23）
兰州市人才创新创业项目(2021-RC-104) （2021-RC-104）
兰州市城关区科技计划(2021-2-9) （2021-2-9）
