中华耳科学杂志2024，Vol.22Issue(1)：136-140,5.DOI:10.3969/j.issn.1672-2922.2024.01.029
模拟长期微重力对大鼠耳蜗基底膜p120-catenin表达的影响
Effects of Simulated Microgravity on P120-catenin Expression in Rat Cochlear Basilar Membrane
摘要
Abstract
Objective To report effects of simulated microgravity on the expression of cochlear catenin cofactor p120-catenin(p120ctn)in the basilar membrane in rats.Methods Healthy male SD rats(n=48)were randomly divided into a simulated microgravity group and a control group(n=24 in each group),which were further divided subgroups based on exposure time(1 day,1 week,8 weeks and 12 weeks,n=6/12 ears in each subgroup.Prior to and immediately after exposure(B0 and P0),distortion product otoacoustic emissions(DPOAEs)and binaural auditory brainstem re-sponse(ABR)thresholds were obtained.At the conclusion of P0 audiometry,rats were executed and both cochleae iso-lated and stained immunohistochemically.Results No differences in DPOAE detection across the groups(P>0.05).ABR thresholds rose as exposure time increased and higher for 1 week,8 weeks and 12 weeks exposure than at B0(P<0.05).Increased P120-catenin expression was noticed in animals with 8 weeks or 12 weeks microgravity exposure as compared to controls(P<0.05).Conclusion Simulated microgravity negatively affects hearing in rats,with p120ctn ex-pression on cochlear basilar membrane increasing along with exposure time.Auditory function impairment brought on by microgravity may be influenced by p120ctn.关键词
微重力/听性脑干反应/黏附连接蛋白/p120-cateninKey words
microgravity/auditory brainstem response/adhesion junction protein/p120-catenin引用本文复制引用
朱莹蕾,吴玮,王刚,周莹,王磊,范博雅,韩浩伦,李保卫,孙喆喆,吴继华..模拟长期微重力对大鼠耳蜗基底膜p120-catenin表达的影响[J].中华耳科学杂志,2024,22(1):136-140,5.基金项目
军队医学科技青年培育计划孵化项目(20QNPY067),航天医学基础与应用国家重点实验开放课题(SMFA19K06) （20QNPY067）
