中华耳科学杂志2024，Vol.22Issue(1)：141-145,5.DOI:10.3969/j.issn.1672-2922.2024.01.030
三组固定方式对应小鼠耳蜗组织染色效果的研究
Effects of Fixation Methods on Cochlear Staining in Mice
摘要
Abstract
Purpose To report effects of fixation techniques on demonstration of general structures and cell mor-phology in the cochlea with preservation of cellular structures and intracellular organelles,to improve current protocols for best immunostaining results.Method One-month-old wild-type C57BL6J mice with normal hearing(n=9)were ran-domly divided into three groups with three mice in each group.Under general anesthesia,animals were perfused with sa-line + 4%paraformaldehyde(PFA)(Group 1)or 4%PFA directly into the heart(Group 2),or decapitated with the co-chlea harvested and directly perfused with 4%PFA.The fixed cochlea was cryosectiond and stained for immunofluores-cence,followed by laser confocal scanning microscopy imaging at low and ultra-high magnification.Results Structures of hair cells and auditory nerves were clearly revealed by all three fixation protocols with similar qualities(P>0.05).However,the structure of stria vascularis was better preserved in group 1 than the other two groups.Conclusions The three fixation methods in this study are all adequate for morphology studies of the organ of Corti and spiral ganglia,while saline + 4%PFA perfusion may be preferable for studying ultrastructure of the stria vascularis.关键词
心脏灌流/耳蜗灌流/Corti's器/血管纹/螺旋神经节Key words
cardiac perfusion/cochlear perfusion/corti's organ/stria vascularis/spiral ganglion引用本文复制引用
位格格,邱士伟,熊巍,袁永一,聂卉一,戴朴..三组固定方式对应小鼠耳蜗组织染色效果的研究[J].中华耳科学杂志,2024,22(1):141-145,5.基金项目
国家自然科学基金(82271185,82271177) （82271185,82271177）
