组织工程与重建外科杂志2024，Vol.20Issue(2)：176-183,198,9.DOI:10.3969/j.issn.1673-0364.2024.02.003
壳聚糖-胶原支架的制备与生物相容性研究
Research on preparation and biocompatibility of chitosan-collagen scaffold
摘要
Abstract
Objective To prepare osteochondral tissue engineering materials by mixing chitosan(CS)and collagen(Col),and their physical properties and biological safety were tested,so as to provide a biological scaffold material for the repair of osteochondral injury.Methods The three groups of mixed scaffolds of CS and Col were made by vacuum freeze-drying method in different proportions.The porosity,water absorption expansion rate,hot water dissolution rate and mechanical properties of the scaffolds were detected.The scaffold structure was observed by scanning electron microscope,and its cytotoxicity and biocompatibility were detected by CCK-8 method,F-actin staining method,and Live/Read cell staining method.Results The scaffolds were white and regular cylinders,and all three groups of scaffolds had good porosity,water absorption expansion rate,hot water dissolution rate and mechanical properties.Scanning electron microscopy showed that all three groups of scaffolds had good porous grid structure,and the physical properties were the best when CS︰Col was 1︰3.The results of CCK-8 method showed that,compared with the control group,there was no obvious growth inhibition in the three groups within a certain time(P>0.05).The results of F-actin staining showed that the cells in each group were uniform in size,the cytoskeleton was regular in shape,and the nucleus was not stained and broken.After DAPI staining,it was found that the cells grew and distributed well in the scaffold.Live/Read cell testing revealed that all three groups of scaffolds had no cytotoxicity to cells,and there was no significant difference in cell viability among the three groups of scaffolds(P>0.05).Conclusion CS-Col composite scaffold has good biocompatibility and physical properties,and it is a potential tissue engineering material for osteochondrosis.关键词
组织工程/骨软骨损伤/胶原/壳聚糖Key words
Tissue engineering/Osteochondral injury/Collagen/Chitosan分类
医药卫生引用本文复制引用
王布雨,唐冬旭,骆艳飞,邓江,阮世强..壳聚糖-胶原支架的制备与生物相容性研究[J].组织工程与重建外科杂志,2024,20(2):176-183,198,9.基金项目
贵州省科技计划项目(黔科合基础-ZK[2021]一般393) （黔科合基础-ZK[2021]一般393）
贵州省卫生健康委科学技术基金项目(gzwkj2021-259) （gzwkj2021-259）
贵州省科技计划项目(黔科合成果-LC[2022]029). （黔科合成果-LC[2022]029）
