中国水产科学2024，Vol.31Issue(1)：70-81,12.DOI:10.12264/JFSC2023-0291
盐度胁迫后青海湖裸鲤Grp78及其相关基因的应答表达
Response expression of Grp78 and its related genes in Gymnocypris przewalskii after salinity stress
摘要
Abstract
Glucose-regulated protein 78 kDa(Grp78)alleviates endoplasmic reticulum stress induced by external stimuli and enhances cell viability.We studied the molecular response mechanisms of Gymnocypris przewalskii to salinity stress.In this study,Grp78 was cloned from G.przewalskii and the response patterns of Grp78 and related genes were analyzed after 5‰,10‰,and 15‰ salinity stress using qPCR.The results showed that the open reading frame length of Grp78 was 1962 bp,encoding 653 amino acids and containing the conserved domain of the HSP70 superfamily.Grp78 was expressed in nine tissues of G.przewalskii,and its expression in the intestinal tract,liver,and heart was significantly higher than that in the other tissues(n=3,P＜0.05).In gills,with the increase in salinity and the extension of stress time,compared with the blank control group,the expressions of Grp78,Hyou1,Prdx1,Nqo1,and UBC were first inhibited and then gradually upregulated,while the expression of DNAJC2,Cu/Zn-SOD,Mn-SOD,and Hmox1 was first upregulated and then gradually downregulated and then upregulated again.In the kidney and liver,the expression of DNAJC2,Cu/Zn-SOD,Mn-SOD,Hmox1,and Nqo1 showed similar patterns of upregulation,downregulation,and upregulation,respectively.These results indicated that Grp78 and its related genes have complex responses to salinity stress and may be involved in adaptation and coping with salinity stress in G.przewalskii.In addition,Grp78 and its related genes—Hyou1,DNAJC2,Hmox1,Nqo1,UBC,Prdx1,Cu/Zn-SOD,and Mn-SOD—participate in the regulation of antioxidant stress in the body and play important roles in reducing salinity damage.关键词
青海湖裸鲤/盐度胁迫/Grp78/基因克隆/基因表达Key words
Gymnocypris przewalskii/salinity stress/Grp78/gene cloning/gene expression分类
农业科技引用本文复制引用
赵静,柳芳,祁洪芳,金文杰,李长忠,王蓉,马淑兄,贾春艳,王璐,勾化宇,李兰英,陈艳霞..盐度胁迫后青海湖裸鲤Grp78及其相关基因的应答表达[J].中国水产科学,2024,31(1):70-81,12.基金项目
国家自然科学基金项目(31460700) （31460700）
青海省科协中青年科技人才托举工程项目(2022QHSKXRCTJ34). （2022QHSKXRCTJ34）
