Identification of novel mammalian viruses in tree shrews(Tupaia belangeri chinensis)OACSTPCD
The Chinese tree shrew(Tupaia belangeri chinensis),a member of the mammalian order Scandentia,exhibits considerable similarities with primates,including humans,in aspects of its nervous,immune,and metabolic systems.These similarities have established the tree shrew as a promising experimental model for biomedical research on cancer,infectious diseases,metabolic disorders,and mental health conditions.Herein,we used metatranscriptomic sequencing to analyze plasma,as well as oral and anal swab samples,from 105 healthy asymptomatic tree shrews to identify the presence of potential zoonotic viruses.In total,eight mammalian viruses with complete genomes were identified,belonging to six viral families,including Flaviviridae,Hepeviridae,Parvovirinae,Picornaviridae,Sedoreoviridae,and Spinareoviridae.Notably,the presence of rotavirus was recorded in tree shrews for the first time.Three viruses-hepacivirus 1,parvovirus,and picornavirus-exhibited low genetic similarity(<70%)with previously reported viruses at the whole-genome scale,indicating novelty.Conversely,three other viruses-hepacivirus 2,hepatovirus A and hepevirus-exhibited high similarity(>94%)to known viral strains.Phylogenetic analyses also revealed that the rotavirus and mammalian orthoreovirus identified in this study may be novel reassortants.These findings provide insights into the diverse viral spectrum present in captive Chinese tree shrews,highlighting the necessity for further research into their potential for crossspecies transmission.
Hong Zhou;Ren-Rong Tian;Xiu-Rong Wang;Jin-Xuan Yang;Yun-Xiao Wang;Ming-Liang Zhao;Xu-Dong Zhang;Yu-Hua Ma;Long-Bao Lv;Edward CHolmes;Yong-Tang Zheng;Wei-Feng Shi
School of Clinical and Basic Medical Sciences,Shandong First Medical University&Shandong Academy of Medical Sciences,Ji’nan,Shandong 250117,China Key Laboratory of Emerging Infectious Diseases in Universities of Shandong,Shandong First Medical University and Shandong Academy of Medical Sciences,Taian,Shandong 271000,ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province,KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases,Center for Biosafety Mega-Science,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan 650223,ChinaSchool of Public Health,Shandong First Medical University&Shandong Academy of Medical Sciences,Ji’nan,Shandong 250117,ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province,KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases,Center for Biosafety Mega-Science,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan 650223,ChinaSchool of Public Health,Shandong First Medical University&Shandong Academy of Medical Sciences,Ji’nan,Shandong 250117,ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province,KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases,Center for Biosafety Mega-Science,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan 650223,ChinaSchool of Public Health,Shandong First Medical University&Shandong Academy of Medical Sciences,Ji’nan,Shandong 250117,ChinaNational Resource Center for Non-Human Primates,Kunming Primate Research Center,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan 650107,ChinaNational Resource Center for Non-Human Primates,Kunming Primate Research Center,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan 650107,ChinaSydney Institute for Infectious Diseases,School of Medical Sciences,The University of Sydney,Sydney,New South Wales 2006,AustraliaKey Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province,KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases,Center for Biosafety Mega-Science,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan 650223,China National Resource Center for Non-Human Primates,Kunming Primate Research Center,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan 650107,ChinaDepartment of Infectious Diseases,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China Shanghai Institute of Virology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China
基础医学
Tree shrew(Tupaia belangeri chinensis)Meta-transcriptomic sequencingMammalian virusesGenomic analysis
《Zoological Research》 2024 (2)
P.429-438,10
supported by the National Key R&D Program of China (2021YFC2300900,2021YFC2301300)Academic Promotion Programme of Shandong First Medical University (2019QL006)Natural Science Foundation of Shandong Province (ZR2020QH274)Yunnan Key Research and Development Program (202103AQ100001,202102AA310055)Key Program of Chinese Academy of Sciences (KJZD-SW-L11)。
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