北华大学学报（自然科学版）2024，Vol.25Issue(3)：319-322,4.DOI:10.11713/j.issn.1009-4822.2024.03.007
基于PD-L1蛋白调控的五味子酚对肝细胞癌细胞增殖的影响
Effects of Schisanhenol on Proliferation of Hepatocellular Carcinoma Cells Regulated by PD-L1 Protein
摘要
Abstract
Objective To investigate the effect of Schisanhenol on the proliferation of hepatocellular carcinoma cells and its related mechanism.Method Hepatocellular carcinoma HepG2 cells were divided into blank control group and three Schisanhenol groups (3μmol/L,10μmol/L and 30μmol/L) .The expressions of PD-L1 proteins in HepG2 cells were detected by Western Blot.Immunofluorescence assay was used to further detect the effect of Schisanhenol on PD-L1 protein.The proliferation of HepG2 cells were observed by cell colony assay.Results The expression results of PD-L1 proteins in HepG2 cells showed that Schisanhenol with 3,10 and 30μmol/L could inhibit the expression of PD-L1 proteins (P<0.05 or P<0.01);The results of immunofluorescence assay and colony assay showed that Schisanhenol with 30 μmol/L could obviously inhibit the proliferation of HepG2 cells (P<0 .01) .Conclusion Schisanhenol inhibits the proliferation of liver cancer cell HepG2 through regulating PD-L1 protein.关键词
五味子酚/肝细胞癌/P D-L1/增殖Key words
Schisanhenol/hepatocellular carcinoma/PD-L1/proliferation分类
医药卫生引用本文复制引用
韩旭,孙靖辉,吴吉平,张志宏,胡雪阳,王玉涵,黄蕾,李梓晴,李思颖,孙海明,王春梅,李贺..基于PD-L1蛋白调控的五味子酚对肝细胞癌细胞增殖的影响[J].北华大学学报（自然科学版）,2024,25(3):319-322,4.基金项目
国家自然科学基金青年基金项目(82204697) （82204697）
吉林省自然科学基金项目(YDZJ202201ZYTS275) （YDZJ202201ZYTS275）
吉林省教育厅科学技术研究项目(JJKH20230086KJ) （JJKH20230086KJ）
北华大学研究生创新计划项目(2022030). （2022030）
