北华大学学报（自然科学版）2024，Vol.25Issue(3)：333-338,6.DOI:10.11713/j.issn.1009-4822.2024.03.009
两色金鸡菊提取物对四氯化碳诱导小鼠急性肝损伤的保护作用
Protective Effect of Coreopsis tinctoria Extract on Acute Liver Injury of Mice Induced by Carbon Tetrachloride
摘要
Abstract
Objective To study the protective effect of Coreopsis tinctoria extract on acute liver injury induced by carbon tetrachloride in mice,and to explore its mechanism.Method The optimum extraction process of Coreopsis tinctoria was obtained by single factor experiment and orthogonal experiment,and the effective components were screened by LC-MS method.The protective effect of Coreopsis tinctoria extract on chemical acute liver injury was investigated by CCl4-induced acute liver injury model in mice.HE staining was used to observe the damage of liver tissue.The changes of ALT,AST,SOD,MDA,TNF-α,IL-6 and IL-1β were detected.Results The results of LC-MS analysis showed that there were 32 main active components in the extract of Core-opsis tinctoria.The results of animal experiments showed that the liver of the model group has obvious pathological changes compared with the blank group,and the detection results of ALT,AST,MDA,SOD,TNF-α,IL-6 and IL-1β were also significantly different (P<0.01).Compared with the model group,the pathological changes and biochemical indexes of the liver in Coreopsis tinctoria administration group improved,among which ALT,AST,TNF-α,IL-6 and IL-1β significantly decreased (P<0.01 or P<0.05),the content of MDA significantly decrea-sed (P<0.01 or P<0.05),and SOD activity significantly increased (P<0.01 or P<0.05).Conclusion Coreopsis tinctoria extract has obvious protective effect on acute liver injury induced by CCl4 in mice,and its mechanism may be related to anti-inflammatory and antioxidant effects.关键词
两色金鸡菊/四氯化碳/急性肝损伤/抗氧化Key words
Coreopsis tinctoria/CCl4/acute liver injury/antioxidation分类
医药卫生引用本文复制引用
李洪宇,曾沛涌,韩迦南..两色金鸡菊提取物对四氯化碳诱导小鼠急性肝损伤的保护作用[J].北华大学学报（自然科学版）,2024,25(3):333-338,6.基金项目
吉林省教育厅科学技术研究项目(JJKH20200078KJ). （JJKH20200078KJ）
