北华大学学报（自然科学版）2024，Vol.25Issue(3)：339-345,7.DOI:10.11713/j.issn.1009-4822.2024.03.010
基于网络药理学和分子对接技术的灵芝改善2型糖尿病作用机制
Ganoderma lucidum Improving Type 2 Diabetes Based on Network Pharmacology and Molecular Docking Technology
摘要
Abstract
Objective The network pharmacology was applied to delve into the mechanisms by which Ganoderma lucidum improves type 2 diabetes mellitus (T2DM).Method Ganoderma lucidum chemical components were curated from TCMSP.SMILE name of the main components were input into SWISS Target Prediction to obtain individual gene name.Disease-related genes were obtained from DisGeNET and GeneCards.Venn database was used to identify intersecting targets.A network of active components and disease targets were constructed on the bioinformatics platform.STRING and Cytoscape databases were employed to build Protein-Protein Interaction (PPI) network.GO and KEGG functional enrichment analyses were conducted by using DAVID database.Molecular docking was performed by using AutoDock database.Results Ganoderma lucidum and T2DM shared 146 common targets.The PPI network comprised 146 nodes and 2201 edges.Five active components of Ganod-erma lucidum and 146 T2DM-related targets were identified.Key active components included Methyl lucidenate F,ganoderic acid A,ganoderic acid E,ganoderic acid A,and Methyl lucidenate Q.Core targets included PGR,PTPN1,NR3C2,etc.KEGG analysis revealed enrichment in pathways such as oocyte meiosis,NF-kappa B signaling pathway,and longevity-regulating pathway.Molecular docking results indicated a strong binding affinity between compounds and important target sites.Conclusion This study identified Methyl lucidenate F,ganoderic acid A,ganoderic acid E,ganoderic acid A,and Methyl lucidenate Q,as core components of Ganoderma lucidum for treating T2DM.By controlling key therapeutic targets such as NR3C2 and HSD11B2,the NF-kappa B binding pathway was affected,thus achieving the therapeutic effects on T2DM.关键词
2型糖尿病/灵芝/网络药理学/NF-kappaB binding通路/分子对接Key words
type 2 diabetes mellitus/Ganoderma lucidum/network pharmacology/NF-kappa B binding pathway/molecular docking分类
医药卫生引用本文复制引用
高德勐,付钰涓,蒲馨怡,徐广宇..基于网络药理学和分子对接技术的灵芝改善2型糖尿病作用机制[J].北华大学学报（自然科学版）,2024,25(3):339-345,7.基金项目
国家自然科学基金项目(81973371). （81973371）
