北华大学学报（自然科学版）2024，Vol.25Issue(3)：346-353,8.DOI:10.11713/j.issn.1009-4822.2024.03.011
基于GEO数据库和转录因子调控网络的结核病药物作用靶点筛选及其作用机制
Screening of Tuberculosis Drug Targets and Their Mechanism Based on GEO Database and Transcription Factor Regulatory Network
摘要
Abstract
Objective To screen anti-tuberculosis drugs and drug targets based on GEO database and transc-ription factor regulatory network.Method To screen differentially expressed genes between TB patients and healthy people through NCBI GEO database.To predict transcription factors in differentially expressed genes through AnimalTFDB 3.0 database,and to construct transcription factor regulatory network and finally to screen related miRNAs through key genes in the regulatory network and to screen key nodes to elucidate initially the molecular mechanism of TB pathogenesis.Results 784 differentially expressed genes were screened through GEO database search.23 transcription factors and the corresponding 790 target genes were screened through AnimalTFDB 3.0 database and the transcription factor-target gene regulatory network was constructed.The miRNAs corresponding to the key nodes were queried through TargetScanHuman 7 .2 ,and the transcription factor-target gene-miRNA regulatory network was constructed to screen four TB drug targets (EP300,CREBBP,ELAVL1,HSP90AA1) and elucidated the regulatory mechanisms between the transcription factors and miRNA.Conclusion The new potential drug targets for TB were screened (EP300,CREBBP,ELAVL1,HSP90AA1),the transcription factors STAT2 can be activated to increase the level of TNF-α and inhibit the expression of miR-9-5p,and are closely related with NOD-like receptor signaling pathway,the pathogenic process of TB were elucidated.关键词
结核病/药物靶点/GEO数据库/转录因子/调控网络Key words
Tuberculosis/drug targets/GEO database/transcription factors/regulatory network分类
医药卫生引用本文复制引用
赵悦含,杨悦,徐广宇..基于GEO数据库和转录因子调控网络的结核病药物作用靶点筛选及其作用机制[J].北华大学学报（自然科学版）,2024,25(3):346-353,8.基金项目
国家自然科学基金项目(81973371). （81973371）
