Journal of Changshu Institute of Technology2024，Vol.38Issue(2)：46-50,5.
3-甲基腺嘌呤对鸡LMH细胞脂代谢的影响机制研究
Effect of 3-Methyl Adenine on Lipid Metabolism in Chicken LMH Cells
李敏 1韦垠 1郁建锋 1徐璐 1顾志良1
作者信息
- 1. 常熟理工学院生物与食品工程学院,江苏常熟 215500
- 折叠
摘要
Abstract
This study focuses on autophagy and liver lipid metabolism in chickens. Firstly, three groups of different concentrations (2.5 mmol/L, 5 mmol/L and 10 mmol/L) were set to treat chicken hepatoma cell lines (LMH) at different concentrations (2.5 mmol/L, 5 mmol/L and 10 mmol/L) for 24 h and 48 h, respectively. The total RNA of the cells was extracted, and the expression of autophagy-related genes at the mRNA level was detected by RT-qPCR to screen the optimal inhibitory concentration and time of 3-MA. Then, the expression of genes related to lipid metabolism in chicken LMH cells treated with optimal conditions 3-MA was detected. The results show that after 24 h treatment of chicken LMH cells with 3-MA at 2.5 mmol/L, the expression of genes Atg3, Map1lc3a and Becn1 was down-regulated at the mRNA level, with the optimal condition being consistent with expectations. Compared with the control group, the expression of genes related to lipid metabolism was significantly down-regulated, the expression level of FASN was significantly up-regulated, and the expression level of Fabp7 and FOXO1 was significantly changed compared with the control group. Therefore, a certain concentration of the autophagy inhibitor 3-MA can promote lipid metabolism in vitro.关键词
3-甲基腺嘌呤/自噬/脂代谢/鸡Key words
3-methyl adenine/autophagy/lipid metabolism/chicken分类
农业科技引用本文复制引用
李敏,韦垠,郁建锋,徐璐,顾志良..3-甲基腺嘌呤对鸡LMH细胞脂代谢的影响机制研究[J].Journal of Changshu Institute of Technology,2024,38(2):46-50,5.
