UHPLC-MS/MS测定化妆品中67种植物提取物标识成分OA北大核心CSTPCD

A new method for the simultaneous and rapid determination of 67 indicative components from plant extracts in cosmetics by ultra high performance liquid chromatography-tandem mass spec-trometry(UHPLC-MS/MS)was established.The cosmetic samples were dispersed with methanol(1%formic acid)and ultrasonic extraction.The filtrate was analyzed by a Agilent RRHD Eclipse Plus Zor-bax C18 chromatographic column(3.0 mm×100 mm,1.8 µm)with mobile phase consisted of 0.1%formic acid aqueous solution and acetonitrile.67 indicative components from plant extracts were ana-lyzed in the electrospray ionization source,including positive ion scanning with multiple reaction monitoring mode(MRM)and negative ion scanning with dynamic multiple reaction monitoring mode(DMRM)respectively.Creams and lotions were choosed as the representative matrix.The 67 indica-tive components showed good linearity with the correlation coefficients(r2)greater than 0.99 in their respective mass concentration ranges.The detection limits(LODs)of 67 indicative components were in the range of 0.0026-1.7 µg/g,and the quantitation limits(LOQs)were in the range of 0.0080-5.0 µg/g.For cream and lotion samples,the average recoveries of 67 indicative components at three different concentration levels ranged of 85.6%-117%and 82.7%-116%,respectively;with relative standard deviations(RSDs,n=6)ranged of 1.0%-12%and 0.90%-12%.The established method is simple,rapid,effective,high sensitive,and is suitable for the qualitative identification and con-tent determination of 67 indicative components from plant extracts in cosmetics.