分析测试学报2024，Vol.43Issue(5)：674-681,8.DOI:10.12452/j.fxcsxb.24010503
氮掺杂Ti3C2 MXene量子点荧光探针用于α-葡萄糖苷酶活性检测
Fluorescent Probe for Detection of α-Glucosidase Activity Based on N-Doped Ti3C2 MXene Quantum Dots
摘要
Abstract
α-Glucosidase is an indispensable enzyme in the glucose metabolism pathway of organ-isms.The development of simple,sensitive and accurate detection methods for α-glucosidase activi-ty and inhibitors screening are of great significance for the treatment and prevention of diabetes.In this work,a novel fluorescent"on-off-on"method for detection of α-glucosidase activity and its in-hibitors screening was developed based on N-doped Ti3C2 MXene quantum dots(N-Ti3C2 MQDs)and internal filtration effect(IFE).The prepared N-Ti3C2 MQDs emitted blue fluorescence(λem = 440 nm)with a fluorescence quantum yield of 15.7%.α-Glucosidase can hydrolyze substrate p-nitrophenyl-α-D-glucopyranoside to produce p-nitrophenol,which causes fluorescence quenching of the N-Ti3C2 MQDs through IFE.After adding the acarbose inhibitor,the activity of α-glucosidase is inhibited and the produced p-nitrophenol by hydrolysis is reduced,resulting in fluorescence recovery of N-Ti3C2 MQDs.Fluorescent intensity of N-Ti3C2 MQDs probe is proportional to α-glucosidase concentra-tion in the range of 5-300 U/L,with a detection limit of 2.5 U/L(S/N = 3).The half-maximal inhibi-tory concentration of inhibitor(IC50)value is calculated to be 178.5 µmol/L for acarbose.This pro-posed method has the advantages of low cost,simple operation,high sensitivity,and good selectivi-ty,which has been successfully used for determination of α-glucosidase activity in human serum.关键词
α-葡萄糖苷酶/氮掺杂Ti3C2 MXene/量子点/荧光探针Key words
α-glucosidase/N-doped Ti3C2 MXene/quantum dots/fluorescent probe分类
化学化工引用本文复制引用
李军,杨新杰,罗焰,李泉,宗玉红,张艳丽,王红斌,杨文荣,庞鹏飞..氮掺杂Ti3C2 MXene量子点荧光探针用于α-葡萄糖苷酶活性检测[J].分析测试学报,2024,43(5):674-681,8.基金项目
国家自然科学基金资助项目(21665027,21565031) （21665027,21565031）
云南省科技厅应用基础研究项目(202001AT070012) （202001AT070012）
