分析测试学报2024，Vol.43Issue(5)：690-702,13.DOI:10.12452/j.fxcsxb.24012601
氮芥与DNA体外加合物及其质谱裂解规律
Mass Spectrometric Fragmentation Patterns of Nitrogen Mustard-DNA Adducts in Vitro
摘要
Abstract
As a dual-use substance,nitrogen mustard is not only on a list of the Convention on the Prohibition of Chemical Weapons,but its derivatives are traditional clinical chemotherapy drugs.In this paper,the adducts formed by incubation of three typical nitrogen mustards(HN1,HN2 and HN3)with 4 bases,4 deoxyribonucleosides,4 deoxyribonucleotides and salmon DNA respectively,were analyzed and identified by high-performance liquid chromatography-quadrupole electrostatic field orbitrap high-resolution mass spectrometry.The HN-DNA adducts in all 39 incubation systems were chromatographically separated and the data were collected by Full MS/dd-MS2 mode to obtain their mass spectrometry characteristics.The results showed that the mono-adducts formed by three kinds of nitrogen mustard with guanine,adenine and cytosine could be identified in each incubation system and the di-adducts formed by nitrogen mustard with guanine or adenine could be identified.Although the mono-adducts formed by three kinds of nitrogen mustard with thymine were detected in the base incubation system,they were not detected in the salmon DNA incubation system,indicat-ing that adducts formed by HNs with thymine base were not primary adducts for DNA.By exploring the mass spectrometry characteristic fragments of each adduct,the specific ion pairs of series of DNA adducts for the multi-reaction monitoring mode of mass spectrometry have been summarized,which lays a foundation for the establishment of a method for structural analysis,accurate traceability and rapid quantification of nitrogen mustard-DNA adducts in complex biological samples.关键词
氮芥/DNA加合物/特征加合离子/高效液相色谱-四极杆静电场轨道阱高分辨质谱Key words
nitrogen mustard/DNA adducts/characteristic fragments/high performance liquid chromatography-quadrupole electrostatic field orbitrap high-resolution mass spectrometry分类
化学化工引用本文复制引用
王朝霞,刘玉龙,陈佳,刘艳芹,徐华,谢剑炜..氮芥与DNA体外加合物及其质谱裂解规律[J].分析测试学报,2024,43(5):690-702,13.基金项目
国家自然科学基金资助项目(21974151) （21974151）
