分析测试学报2024，Vol.43Issue(5)：714-721,8.DOI:10.12452/j.fxcsxb.24010301
基于适配体-MOF模拟酶比色检测bPAG9
A Colorimetric Detection of bPAG9 Based on Aptamer and Peroxi-dase-like Mimics of Metal Organic Framework
摘要
Abstract
The aim of this study is to establish a new colorimetric method for the detection of bovine pregnancy associated glycoprotein 9(bPAG9)based on the metal-organic framework Fe-MIL-101 and double aptamer sandwich principle.In a typical experimental procedure,biotinylated aptamer1 was immobilized onto microplate to act as capture probes.Fe-MIL-101 functionalized aptamer 2 act as a signal probe.In the presence of bPAG9,aptamer 1 and aptamer 2 bind with bPAG9 and forma double aptamer sandwich structure on solid phase carrier.Fe-MIL-101 coupled to aptamer 2 enable to catalyze the colorless peroxidase substrate to yield a colorimetric output signal.Thus,the qualita-tive and quantitative analysis of bPAG9 could be realized by the change of colorimetric signal.The re-sults showed that under the optimal conditions,the linear detection range were 0.5-50 ng/mL for bPAG9 in buffered solution,with a limit of detection(LOD)of 0.13 ng/mL.The average recoveries of bPAG9 in blank serum samples were in the range of 89.4%-104%,and the relative standard devia-tions were less than 10%.Subsequently,the serum samples from Holstein cows at 28 days after in-semination were analyzed by the developed assay.The test results were confirmed with ultrasonogra-phy and compared with PAG-ELISA test kit.The diagnostic sensitivity,specificity,and accuracy of the developed assay were 92.5%,90.0%and 91.5%,respectively.The developed assay has such advantages as simple,noninvasive,and economical.It has a good application prospect in the field of cow early pregnancy detection.关键词
妊娠相关糖蛋白/适配体/金属有机框架/妊娠诊断/奶牛Key words
pregnancy associated glycoprotein/aptamer/metal organic framework/pregnancy di-agnosis/cow分类
化学化工引用本文复制引用
卢春霞,刘长彬,张振良,卢守亮,吴世嘉..基于适配体-MOF模拟酶比色检测bPAG9[J].分析测试学报,2024,43(5):714-721,8.基金项目
兵团重点领域科技计划(2023AB009-01) （2023AB009-01）
